Paper · 2000 · Acta Phytopathologica Sinica

Molecular marker assisted identification of Pm genes involved in the powdery mildew resistant wheat cultivars (lines).

Liu Jinyuan; Tao Wen-Jing; Duan XiaYu; et al.

6
claims
12
verbatim quotes
3
citations
4
research areas
—
Not open access

Abstract

The Pm genes contained in wheat cultivars (lines) developed by Cytogenetics Institute of Nanjing Agricultural University in corporation with Lixiahe Agricultural Research Institute of Jiangsu Province and those from other sources were identified using RFLP markers tightly linked to Pm2 and Pm4a genes. The Pm genes identified in most of the tested materials by molecular markers were in accordance with these by pedigree deduction and analysis of resistance spectrum. But, the statements previously made about “Baimian No.3”, “Kenguia No.1” and “Zhengzhou 831” containing “Pm2x”, “KG” (6A) and Pm2+6 respectively, were not coincided with the results based on the molecular marker analysis which showed that all above mentioned three cultivars contain Pm4 gene, instead of Pm2 gene. The results were also in agreement with that from resistance spectrum analysis. Two cultivars used as Pm6 differential hosts Timgalen and CI12632/8Cc introduced from IWWPMN, in which the resistance was degraded, were confirmed the Pm6 gene had been lost by RFLP analysis.

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Claims 6

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Most marker identifications of Pm genes agreed with pedigree deduction and resistance spectrum analysis.

Gene
Pm genese0
Outcome
in accordance with these by pedigree deduction and analysis of resistance spectrume0
Comparator
pedigree deduction and analysis of resistance spectrume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The Pm genes identified in most of the tested materials by molecular markers were in accordance with these by pedigree deduction and analysis of resistance spectrum”

✓ located verbatim in the source · abstract, characters 303–467 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Baimian No.3 carried Pm4 rather than the previously reported Pm2x.

Material
Baimian No.3e0
Gene
Pm4 genee0
Outcome
contain Pm4 gene, instead of Pm2 genee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

““Baimian No.3”, “Kenguia No.1” and “Zhengzhou 831” containing “Pm2x”, “KG” (6A) and Pm2+6 respectively, were not coincided with the results based on the molecular marker analysis which showed that all above mentioned three cultivars contain Pm4 gene, instead of Pm2 gene”

✓ located verbatim in the source · abstract, characters 511–781 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Kenguia No.1 carried Pm4 rather than its previous assignment.

Material
Kenguia No.1e0
Gene
Pm4 genee0
Outcome
contain Pm4 gene, instead of Pm2 genee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

““Baimian No.3”, “Kenguia No.1” and “Zhengzhou 831” containing “Pm2x”, “KG” (6A) and Pm2+6 respectively, were not coincided with the results based on the molecular marker analysis which showed that all above mentioned three cultivars contain Pm4 gene, instead of Pm2 gene”

✓ located verbatim in the source · abstract, characters 511–781 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zhengzhou 831 carried Pm4 rather than its previous assignment.

Material
Zhengzhou 831e0
Gene
Pm4 genee0
Outcome
contain Pm4 gene, instead of Pm2 genee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

““Baimian No.3”, “Kenguia No.1” and “Zhengzhou 831” containing “Pm2x”, “KG” (6A) and Pm2+6 respectively, were not coincided with the results based on the molecular marker analysis which showed that all above mentioned three cultivars contain Pm4 gene, instead of Pm2 gene”

✓ located verbatim in the source · abstract, characters 511–781 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

RFLP analysis confirmed loss of Pm6 in Timgalen.

Material
Timgalene0
Gene
Pm6 genee0
Outcome
Pm6 gene had been loste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Two cultivars used as Pm6 differential hosts Timgalen and CI12632/8Cc introduced from IWWPMN, in which the resistance was degraded, were confirmed the Pm6 gene had been lost by RFLP analysis”

✓ located verbatim in the source · abstract, characters 863–1053 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

RFLP analysis confirmed loss of Pm6 in CI12632/8Cc.

Material
CI12632/8Cce0
Gene
Pm6 genee0
Outcome
Pm6 gene had been loste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Two cultivars used as Pm6 differential hosts Timgalen and CI12632/8Cc introduced from IWWPMN, in which the resistance was degraded, were confirmed the Pm6 gene had been lost by RFLP analysis”

✓ located verbatim in the source · abstract, characters 863–1053 · show in text ↑
t0

“wheat”

✓ located verbatim in the source · title, characters 94–99 · show in text ↑