Claim · model-written summary
The genome-wide approach significantly reduced experiment design time, including PCR validation, with one-time computation overhead.
Verbatim
e0“The genome-wide approach provides a significant decrease in the time required to design a CRISPR experiment, including validation through PCR, at the expense of an overhead compute time required once per genome, at the first run.”
t0“crop”
t1“crop”
Title
CROPSR: an automated platform for complex genome-wide CRISPR gRNA design and validation
Hans Müller Paul; Dave D. Istanto; Jacob Heldenbrand; et al. BMC Bioinformatics 2022. doi:10.1186/s12859-022-04593-2
Other claims from this paper (3)
- Method / resource CROPSR supplies genome-wide gRNA design and evaluation.
- Method / resource The scoring model can give scores in repetitive, A/T-rich regions where existing tools sometimes failed.
- Method / resource The gRNA scoring model significantly improved prediction accuracy over existing tools, even for non-crop genomes.