Claim · model-written summary
Catalytic activity of engineered PIP5K3 variants was essential for their physiological function in restoring root hair formation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- engineered PIP5K3 variantse6
- Gene
- PIP5K3e6
- Outcome
- physiological functione6
- Qualifier
- catalytic activitye6
Not reportedComparatorDirection
Verbatim
t0“Root Hair Formation inArabidopsis thaliana”
e6“Using reestablishment of root hair formation in T-DNA mutants as a bioassay for physiological functionality of engineered PIP5K3 variants, catalytic activity was found to be essential for physiological function”
Title
Irene Stenzel; Till Ischebeck; Sabine König; et al. The Plant Cell 2008. doi:10.1105/tpc.107.052852
Other claims from this paper (9)
- Observation PIP5K3 is expressed in the root cortex, epidermal cells and root hairs.
- Observation Recombinant PIP5K3 protein was catalytically active and converted phosphatidylinositol-4-phosphate to PtdIns(4,5)P2.
- Intervention effect Homozygous T-DNA-disrupted PIP5K3 mutant plants were compromised in root hair formation.
- Intervention effect Expressing wild-type PIP5K3 cDNA under a 1500-bp PIP5K3 promoter fragment complemented the root hair formation phenotype of the mutants.
- Intervention effect Root hair-specific PIP5K3 overexpression caused root hair deformation and loss of cell polarity, with increasing accumulation of PIP5K3 transcript.
- Intervention effect The N-terminal domain with membrane occupation and recognition nexus repeats, which is not required for catalytic activity, is essential for establishing root hair growth.
- Observation Fluorescence-tagged PIP5K3 localized to the periphery of the apical region of root hair cells, possibly associating with the plasma membrane and/or exocytotic vesicles.
- Intervention effect Transient expression of full-length PIP5K3 in tobacco pollen tubes increased plasma membrane association of a PtdIns(4,5)P2-specific reporter.
- Mechanism Root hair development requires PIP5K3-dependent PtdIns(4,5)P2 production in the apical region of root hair cells.