Claim · model-written summary
Chicory 1-FEH I cDNA was cloned and shown functional by expression in potato tubers.
- Taxon
- chicory
Cichorium intybuse0 - Gene
- 1‐FEH Ie0
- Outcome
- Functionality of the cDNAe1
Not reportedComparatorValue
Verbatim
e0“This paper describes the cloning and functional analysis of chicory ( Cichorium intybus L.) fructan 1‐exohydrolase I cDNA ( 1‐FEH I ).”
e1“Functionality of the cDNA was demonstrated by heterologous expression in potato tubers.”
Title
Wim Van den Ende; An Michiels; Joke De Roover; et al. The Plant Journal 2000. doi:10.1111/j.1365-313x.2000.00890.x
Other claims from this paper (7)
- Method / resource The cloned chicory enzyme is described as the first plant FEH cloned.
- Observation Chicory 1-FEH I occupies a distinct phylogenetic position and is more homologous to cell-wall invertases (44–53%) than vacuolar invertases (38–41%) or fructosyl transferases (33–38%).
- Observation Chicory 1-FEH I was not purified from apoplastic fluid above levels explained by cellular leakage.
- Observation The data suggest vacuolar localization of chicory 1-FEH I.
- Observation The pI of chicory 1-FEH I was 6.5, lower than expected for a typical cell-wall invertase.
- Mechanism The authors propose that chicory 1-FEH I evolved from a cell-wall invertase-like ancestor that acquired a vacuolar targeting signal.
- Observation Chicory root 1-FEH I mRNA increased through autumn, especially during low-temperature storage.