Claim · model-written summary
Enrichment decreased potato host genomic reads by about 1.7-fold.
Solanum tuberosumVerbatim
e0“a ∼1.7-fold decrease in host genomic reads compared to the DNA extracted from the same sample without density gradient centrifugation enrichment.”
e1“potato infected with a ‘ Candidatus Phytoplasma australasia’-related strain”
Title
Bianca Rodrigues Jardim; Lucy T. T. Tran-Nguyen; Cherie Gambley; et al. Frontiers in Microbiology 2022. doi:10.3389/fmicb.2022.937648
Other claims from this paper (9)
- Method / resource Enrichment increased phytoplasma HTS reads from infected potato by about 14-fold.
- Method / resource Enrichment increased potato phytoplasma assembly size by about 15,000 bp.
- Method / resource Enrichment increased potato phytoplasma assembly N50 by about 14,800 bp.
- Method / resource Enrichment produced about threefold more phytoplasma reads in capsicum.
- Method / resource Enrichment produced about twofold fewer host genomic reads in capsicum.
- Method / resource Enrichment increased capsicum phytoplasma genome assembly size by about 137,000 bp.
- Method / resource Enrichment increased capsicum phytoplasma assembly N50 by about 4,000 bp.
- Method / resource Phytoplasmas from potato and capsicum were enriched at a density of 1.049–1.058 g/ml.
- Method / resource The authors provide two contiguous phytoplasma reference genomes from infected Solanaceae hosts.