Claim · model-written summary
Genetic analysis indicated that the virescent leaf trait is controlled by one single recessive gene, v-2.
- Taxon
- Cucumber
Cucumis sativust0 - Gene
- v-2e2
- Outcome
- virescent leaf was controlled by one single recessive genee2
Not reportedDirection
Verbatim
t0“Cucumber”
e2“the virescent leaf was controlled by one single recessive gene v-2”
Title
Fine Mapping and Transcriptome Analysis of Virescent Leaf Gene v-2 in Cucumber (Cucumis sativus L.)
Kaijing Zhang; Ying Li; Wenwei Zhu; et al. Frontiers in Plant Science 2020. doi:10.3389/fpls.2020.570817
Other claims from this paper (8)
- Observation Cucumber mutant 104Y, arising by spontaneous mutation, had yellow cotyledons and upper five true leaves.
- Observation The yellow true leaves of 104Y gradually turned green from top to bottom with increased chlorophyll contents.
- Observation The v-2 gene was mapped into a 36.0-39.7 Mb interval on chromosome 3 using BSA-seq and linkage analysis.
- Observation Fine mapping with BC1 and F2 populations narrowed v-2 to a 73-kb genomic region containing 8 genes.
- Observation Only one nonsynonymous mutation was found in the Csa3G890020 gene, which encodes an auxin F-box protein, and it was predicted to be the candidate gene for virescent leaf.
- Observation Csa3G890020 expression level did not change between EC1 and mutant 104Y.
- Observation RNA-seq showed that key genes in chlorophyll biosynthesis and the auxin signaling transduction network were mainly down-regulated in 104Y compared with EC1.
- Mechanism The authors suggest the regulatory function of Csa3G890020 could act at the post-transcriptional rather than the transcriptional level.