Abstract
Leaf color mutants are the ideal materials to explore the pathways of chlorophyll metabolism, chloroplast development and photosynthesis system. In this study, a new virescent leaf mutant 104Y was identified by spontaneous mutation, whose cotyledon and upper five true leaves were yellow color. The yellow true leaves gradually turned green from top to bottom with increased chlorophyll contents. Genetic analysis indicated that the virescent leaf was controlled by one single recessive gene v-2, which was accurately mapped into 36.0-39.7 Mb interval on chromosome 3 by using BSA-seq and linkage analysis. Fine mapping analysis further narrowed v-2 into 73-kb genomic region including 8 genes with BC1 and F2 populations. Through BSA-seq and cDNA sequencing analysis, only one nonsynonymous mutation existed in the Csa3G890020 gene encoding auxin F-box protein was identified, which was predicted as the candidate gene controlling virescent leaf. Comparative transcriptome analysis and quantitative real-time PCR analysis revealed that the expression level of Csa3G890020 was not changed between EC1 and 104Y. However, RNA-seq analysis identified that the key genes involved in chlorophyll biosynthesis and auxin signaling transduction network were mainly down-regulated in 104Y compared with EC1, which indicated that the regulatory functions of Csa3G890020 could be performed at post-transcriptional level rather than transcriptional level. This is the first repot to map-based clone an auxin F-box protein gene related to virescent leaf in cucumber. The results will exhibit a new insight into the chlorophyll biosynthesis regulated by auxin signaling transduction network.
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Claims 9
Claim · model-written summary
Cucumber mutant 104Y, arising by spontaneous mutation, had yellow cotyledons and upper five true leaves.
- Taxon
- Cucumber
Cucumis sativust0 - Material
- 104Ye0
- Outcome
- cotyledon and upper five true leaves were yellow colore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“a new virescent leaf mutant 104Y was identified by spontaneous mutation, whose cotyledon and upper five true leaves were yellow color”
Claim · model-written summary
The yellow true leaves of 104Y gradually turned green from top to bottom with increased chlorophyll contents.
- Taxon
- CucumberCucumis sativust0
- Outcome
- chlorophyll contentse1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e1“The yellow true leaves gradually turned green from top to bottom with increased chlorophyll contents”
Claim · model-written summary
Genetic analysis indicated that the virescent leaf trait is controlled by one single recessive gene, v-2.
- Taxon
- CucumberCucumis sativust0
- Gene
- v-2e2
- Outcome
- virescent leaf was controlled by one single recessive genee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e2“the virescent leaf was controlled by one single recessive gene v-2”
Claim · model-written summary
The v-2 gene was mapped into a 36.0-39.7 Mb interval on chromosome 3 using BSA-seq and linkage analysis.
- Taxon
- CucumberCucumis sativust0
- Outcome
- mapped into 36.0-39.7 Mb interval on chromosome 3e3
- Value
- 36.0-39.7 Mbe3
Not reportedGeneDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e3“accurately mapped into 36.0-39.7 Mb interval on chromosome 3 by using BSA-seq and linkage analysis”
Claim · model-written summary
Fine mapping with BC1 and F2 populations narrowed v-2 to a 73-kb genomic region containing 8 genes.
- Taxon
- CucumberCucumis sativust0
- Gene
- v-2e4
- Outcome
- narrowed v-2 into 73-kb genomic region including 8 genese4
- Value
- 73-kbe4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e4“Fine mapping analysis further narrowed v-2 into 73-kb genomic region including 8 genes with BC1 and F2 populations”
Claim · model-written summary
Only one nonsynonymous mutation was found in the Csa3G890020 gene, which encodes an auxin F-box protein, and it was predicted to be the candidate gene for virescent leaf.
- Taxon
- CucumberCucumis sativust0
- Gene
- Csa3G890020e5
- Outcome
- only one nonsynonymous mutatione5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e5“only one nonsynonymous mutation existed in the Csa3G890020 gene encoding auxin F-box protein was identified”
Claim · model-written summary
Csa3G890020 expression level did not change between EC1 and mutant 104Y.
- Taxon
- CucumberCucumis sativust0
- Material
- 104Ye6
- Gene
- Csa3G890020e6
- Outcome
- expression levele6
- Direction
- not changede6
- Comparator
- EC1e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e6“the expression level of Csa3G890020 was not changed between EC1 and 104Y”
Claim · model-written summary
RNA-seq showed that key genes in chlorophyll biosynthesis and the auxin signaling transduction network were mainly down-regulated in 104Y compared with EC1.
- Taxon
- CucumberCucumis sativust0
- Material
- 104Ye7
- Outcome
- key genes involved in chlorophyll biosynthesis and auxin signaling transduction networke7
- Direction
- down-regulatede7
- Comparator
- EC1e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e7“the key genes involved in chlorophyll biosynthesis and auxin signaling transduction network were mainly down-regulated in 104Y compared with EC1”
Claim · model-written summary
The authors suggest the regulatory function of Csa3G890020 could act at the post-transcriptional rather than the transcriptional level.
- Taxon
- CucumberCucumis sativust0
- Gene
- Csa3G890020e0
- Outcome
- regulatory functionse0
- Qualifier
- post-transcriptional level rather than transcriptional levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the regulatory functions of Csa3G890020 could be performed at post-transcriptional level rather than transcriptional level”