Claim · model-written summary
Comparative sequences support both introns and V0.
- Taxon
- grapevine (Vitis vinifera)
Vitis viniferae1 - Interactor
- GRBVe0pathogen
- Gene
- V0e0
- Outcome
- existence of both introns and V0e0
Verbatim
e0“Comparative analyses of full-length GRBV sequences and the related tentative grabloviruses Prunus geminivirus A and wild Vitis virus 1 support the existence of both introns and V0”
e1“GRBV-infected grapevine (Vitis vinifera)”
Title
Evidence for the splicing of grablovirus transcripts reveals a putative novel open reading frame
José Vargas-Asencio; Harris Liou; Keith L. Perry; et al. Journal of General Virology 2019. doi:10.1099/jgv.0.001234
Other claims from this paper (7)
- Observation RNA sequencing of infected grapevine confirmed the predicted GRBV C1-C2 intron.
- Observation RNA sequencing identified a larger GRBV virion-sense intron spanning V2.
- Observation Public datasets supported both GRBV introns across isolates.
- Observation RT-PCR supported both introns in infected grapevine.
- Observation Transient expression in Nicotiana benthamiana supported the intron observations.
- Observation The GRBV virion-sense donor site lies within V0 and V02 and the acceptor lies in V2.
- Observation Splicing is predicted to remove the V2 protein N terminus.