Claim · model-written summary
RT-PCR supported both introns in infected grapevine.
VitisNot reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These observations were further supported by RT-PCR analyses in both GRBV-infected grapevine”
Title
Evidence for the splicing of grablovirus transcripts reveals a putative novel open reading frame
José Vargas-Asencio; Harris Liou; Keith L. Perry; et al. Journal of General Virology 2019. doi:10.1099/jgv.0.001234
Other claims from this paper (7)
- Observation RNA sequencing of infected grapevine confirmed the predicted GRBV C1-C2 intron.
- Observation RNA sequencing identified a larger GRBV virion-sense intron spanning V2.
- Observation Public datasets supported both GRBV introns across isolates.
- Observation Transient expression in Nicotiana benthamiana supported the intron observations.
- Observation The GRBV virion-sense donor site lies within V0 and V02 and the acceptor lies in V2.
- Observation Splicing is predicted to remove the V2 protein N terminus.
- Observation Comparative sequences support both introns and V0.