Claim · model-written summary
P. brasiliense was re-isolated from inoculated avocado fruits, fulfilling Koch’s postulates.
- Taxon
- avocado
Persea americanae0
- Interactor
- P. brasiliensePectobacterium brasiliensee4pathogen
- Outcome
- re-isolatede4
- Qualifier
- fulfilling Koch’s postulatese4
Verbatim
e0“To prove pathogenicity, strain P22-98 was grown on LB for 48 h at 28 o C, suspended in sterile water at 1-2 x 10 6 CFU/ml, and injected into six mature avocado fruits using a sterile 31G BD insulin syringe”
e4“Bacterial colonies were consistently re-isolated from the bacterial-inoculated fruits and morphologically (LB) and molecularly (16s rRNA, Pec.dnaA) identified as P. brasiliense, thus fulfilling Koch’s postulates”
Title
First Report of Pectobacterium brasiliense Causing Soft Rot on Avocado in Hawai‘i, USA
Lionel Sugiyama; Eva Brill; MaryAnn Villalun; et al. Plant Disease 2026. doi:10.1094/pdis-01-26-0102-pdn
Other claims from this paper (5)
- Intervention effect Pectobacterium brasiliense strain P22-98 injected into avocado fruits produced discoloured, water-soaked, soft and sunken lesions within four days, unlike sterile-water controls.
- Observation Sterile-water-inoculated avocado fruits remained firm and odour-free.
- Observation The partial 16S rRNA sequence of the isolate was 99.84% identical to the type strain of Pectobacterium brasiliense.
- Observation The DnaA sequence of the isolate was 99.53% identical to P. brasiliense FP220416.
- Observation P. brasiliense caused postharvest bacterial soft rot of avocado in Hawai‘i, the first report for this disease.