A. eucalyptorum 9 claims

Claim · model-written summary

Apoharknessia eucalyptorum is reported for the first time as causing leaf blight on E. grandis x E. urophylla in China.

Taxon

E. grandis × E. urophyllaEucalyptus grandise1

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

leaf blighte1

Setting

Chinae1

Verbatim

e1

“this is the first report of A. eucalyptorum causing leaf blight on E. grandis × E. urophylla in China”

Claim · model-written summary

Inoculated leaves developed lesions, whereas control leaves showed only browning at the inoculation point.

Taxon

E. grandis × E. urophyllaEucalyptus grandise1

Treatment

conidia solution (1×107 conidia/ml)e3

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

Lesionse2

Comparator

control leavese2

Not reportedDirection

Verbatim

e1

“this is the first report of A. eucalyptorum causing leaf blight on E. grandis × E. urophylla in China”

e2

“Lesions appeared on all the pathogen-inoculated leaves, whereas only the inoculation point turned brown on the control leaves”

e3

“conidia solution (1×107 conidia/ml) and sterile water were sprayed to leaves in different groups”

Claim · model-written summary

The pathogen was identified as A. eucalyptorum from morphological and molecular characteristics.

Taxon

E. grandis × E. urophyllaEucalyptus grandise1

Interactor

A. eucalyptorumApoharknessia eucalyptorume4pathogen

Outcome

identifiede4

Verbatim

e1

“this is the first report of A. eucalyptorum causing leaf blight on E. grandis × E. urophylla in China”

e4

“Thus, based on morphological (Crous et al. 2017; Garrett et al. 2018) and molecular characteristics, the pathogen was identified as A. eucalyptorum”

Claim · model-written summary

Mycelial growth of A. eucalyptorum was best at 25°C on PDA, BEAN and MEA.

Taxon

E. dunniiEucalyptus dunniie1

Treatment

25°C on PDA, BEAN and MEAe2

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

mycelial growthe2

Direction

beste2

Not reportedComparator

Verbatim

e1

“leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media”

e2

“The best conditions for mycelial growth were at 25°C on PDA, BEAN and MEA”

Claim · model-written summary

Sporulation of A. eucalyptorum was optimal at 25°C on all tested media.

Taxon

E. dunniiEucalyptus dunniie1

Treatment

25°C for all tested mediae2

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

sporulatione2

Direction

optimale2

Not reportedComparator

Verbatim

e1

“leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media”

e2

“Considering sporulation, optimal conditions were 25°C for all tested media and 20°C for PDA and BEAN”

Claim · model-written summary

Sporulation of A. eucalyptorum was optimal at 20°C on PDA and BEAN.

Taxon

E. dunniiEucalyptus dunniie1

Treatment

20°C for PDA and BEANe2

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

sporulatione2

Direction

optimale2

Not reportedComparator

Verbatim

e1

“leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media”

e2

“Considering sporulation, optimal conditions were 25°C for all tested media and 20°C for PDA and BEAN”

Claim · model-written summary

Colony characteristics of A. eucalyptorum changed with temperature, with fluffy mycelium at 15°C.

Taxon

E. dunniiEucalyptus dunniie1

Treatment

temperaturee2

Interactor

A. eucalyptorumApoharknessia eucalyptorume1pathogen

Outcome

Colony characteristicse2

Qualifier

at 15°C colonies formed a fluffy myceliume2

Verbatim

e1

“leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media”

e2

“Colony characteristics changed with temperature; at 15°C colonies formed a fluffy mycelium, whereas at 25°C mycelium spread across the medium forming dark margins lined by dirty-white mycelium and conidia”

Claim · model-written summary

The authors indicate MEA, PDA and BEAN at 25°C as conditions for in vitro growth and sporulation of A. eucalyptorum.

Taxon

E. dunniiEucalyptus dunniie1

Treatment

the culture media MEA, PDA and BEAN at 25°Ce2

Interactor

A. eucalyptorumApoharknessia eucalyptorume2pathogen

Outcome

in vitro growth and sporulatione2

Verbatim

e1

“leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media”

e2

“The conditions indicated for in vitro growth and sporulation of A. eucalyptorum are the culture media MEA, PDA and BEAN at 25°C”

Claim · model-written summary

Inoculation and reisolation confirmed Eucalyptus dunnii hosts this leaf blight.

Taxon

Eucalyptus dunniiEucalyptus dunniie0

Interactor

A. eucalyptorumApoharknessia eucalyptorume0pathogen

Outcome

is a host for this leaf blighte0

Verbatim

e0

“Inoculation tests and re‐isolation of A. eucalyptorum confirmed that Eucalyptus dunnii is a host for this leaf blight”