Claim · model-written summary
The 16S rDNA sequence of the strains showed 99% identity to Pcb isolates from cucumber, pepper, cabbage and potato.
- Taxon
- squash and watermelont0
- Interactor
- Pcbe2pathogen
- Outcome
- 16S rDNA sequence analysise2
- Value
- 99% identitye2
- Comparator
- Pcb isolated from cucumbere2
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentSettingDirectionQualifier
Verbatim
t0“First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia”
e2“The 16S rDNA sequence analysis (GenBank nos. MH031784, MH031785, and MH031786) showed 99% identity to the sequences of Pcb isolated from cucumber (CP020350)”
Title
N. Zlatković; A. Prokić; K. Gašić; et al. Plant Disease 2019. doi:10.1094/pdis-12-18-2213-pdn
Other claims from this paper (12)
- Observation Pectobacterium carotovorum subsp. brasiliense was first reported causing soft rot on squash and watermelon in Serbia.
- Observation The recA sequences of three strains showed the highest nucleotide identity (100 to 98.35%) with Pcb sequences in GenBank.
- Observation The gapA sequences of three strains showed the highest nucleotide identity (99.42 to 98.08%) with Pcb sequences in GenBank.
- Intervention effect Spray inoculation with the strains caused water-soaked lesions on watermelon and melon seedlings, but not on water-sprayed controls.
- Intervention effect Inoculation by pricking caused soft rot within 48 h on zucchini fruits, but not on sterile-water controls.
- Observation Bacteria reisolated from inoculated plant and fruit tissue were identified as Pcb by PCR, completing Koch’s postulates.
- Observation Round, water-soaked lesions were observed on fruits of sporadic squash plants in two breeder variety trial fields in Bački Petrovac.
- Observation Symptomatic squash plants of cultivar GL Maxima showed 30% symptom intensity.
- Observation Vine infection was observed on watermelon plants in a commercial field in Čelarevo.
- Observation Soft rot lesions on infected watermelon stems led to collapse and wilting of entire vines.
- Observation Fewer than 10% of watermelon plants showed symptoms.
- Observation The strains caused soft rot on potato tuber slices.