Claim · model-written summary
The optimal Taq polymerase concentration for the SRAP system is 0.02 U/μl.
- Taxon
- Carthamus tinctorius L.
Carthamus tinctoriuse0 - Outcome
- Taq polymerasee1
- Value
- 0.02 U/μle1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A SRAP reaction system for Carthamus tinctorius L.was established”
e1“In a 25 μl reaction system,Taq polymerase was 0.02 U/μl,dNTP was 0.25 mmol/L,Primer was 0.30 μmol/L,and Mg~(2+) was 3.0 mmol/L”
Title
Peng Sa; Guo Mei-li; Yuehua Chen; et al. Academic Journal of Second Military Medical University 2006
Other claims from this paper (8)
- Method / resource An SRAP reaction system was established for Carthamus tinctorius L. in a 25 μl reaction volume.
- Method / resource The optimal dNTP concentration for the SRAP system is 0.25 mmol/L.
- Method / resource The optimal primer concentration for the SRAP system is 0.30 μmol/L.
- Method / resource The optimal Mg2+ concentration for the SRAP system is 3.0 mmol/L.
- Intervention effect After optimization, target bands increased in the SRAP amplification.
- Observation The optimized SRAP system gave good reproducibility.
- Observation The SRAP amplification results were satisfactory.
- Recommendation The SRAP reaction system is recommended as suitable for analysis of Carthamus tinctorius L.