Claim · model-written summary
Petiole explants cultured on DKW medium with 2.2 ug/L TDZ during callus induction gave rapid and efficient transformation of this hybrid poplar genotype.
PopulusNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of petiole explants cultured on DKW medium along with 2.2 ug/L TDZ during the callus induction phase resulted in rapid and efficient transformation of this hybrid poplar genotype”
t0“Hybrid Poplar”
Title
An Efficient High-Throughput Genetic Transformation Method for Hybrid Poplar
Gary Coleman; Gen Li; Reuben Tayengwa; et al. Forests 2026. doi:10.3390/f17040445
Other claims from this paper (4)
- Method / resource Petiole explants from in vitro-cultured hybrid poplar plantlets regenerated shoots comparably to stem or leaf explants.
- Intervention effect Petiole explants of hybrid poplar cultured on DKW medium showed significantly greater tissue growth than on MS medium.
- Intervention effect Including low concentrations of thidiazuron in callus-inducing media significantly enhanced shoot regeneration competence of hybrid poplar petiole explants.
- Method / resource Transformation efficiencies of 70% to 90% were obtained when the method was applied to activation tagging or CRISPR-Cas9 and Cas12a gene editing.