Claim · model-written summary
NbGRP-YFP shows a punctate distribution at the cell wall boundaries.
- Taxon
- N. benthamiana
Nicotiana benthamianae2 - Gene
- NbGRPe3
- Outcome
- punctate distribution pattern at the cell wall boundariese3
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“we present an experimental strategy involving the genetic editing of an antiviral cell wall component in N. benthamiana”
e3“Confocal microscopy revealed that the NbGRP protein fused to yellow fluorescent protein (NbGRP-YFP) displayed a punctate distribution pattern at the cell wall boundaries”
Title
Zhu Fang; Xinru Zhao; Min Du; et al. Plant Biotechnology Journal 2025. doi:10.1111/pbi.70089
Other claims from this paper (11)
- Association NbGRP mRNA was upregulated threefold in response to ToBRFV infection.
- Observation NbGRP is a plasmodesmata-localized protein, as its fluorescence remained in the cell wall after plasmolysis.
- Intervention effect Transient overexpression of NbGRP significantly inhibited the RNA and protein levels of ToBRFV.
- Intervention effect TRV-induced silencing of NbGRP raised GFP fluorescence, viral genomic RNA and ToBRFV coat protein levels relative to TRV-GUS plants.
- Intervention effect NbGRP mutants have enlarged leaves and a 100% increase in biomass compared with wild-type plants.
- Intervention effect When infiltrated with ToBRFV-GFP, NbGRP-edited plants show significantly higher ToBRFV RNA and protein levels.
- Intervention effect When infiltrated with ToBRFV-GFP, NbGRP-edited plants show increased GFP expression.
- Intervention effect NbGRP mutants show enhanced infection by TMV-GFP and PVX-GFP, with GFP accumulation increasing approximately twofold compared with wild-type plants.
- Intervention effect The NbGRP mutant shows an approximately twofold increase in GFP nanobody accumulation from TMV-GFPNano compared with wild-type plants.
- Intervention effect The recovered GFP nanobody is significantly increased in the NbGRP mutant compared with wild-type plants.
- Observation The purified GFP nanobody can be used to detect GFP.