Galactinol was imported into Agrobacterium through MelB with nanomolar affinity.

Claim · model-written summary

Galactinol was imported into Agrobacterium through MelB with nanomolar affinity.

Taxon

tomato plantsSolanum lycopersicume0

Interactor

AgrobacteriumAgrobacteriume0pathogen

Gene

MelBe0

Outcome

galactinol, recently described as a plant defense molecule, is imported into Agrobacterium via MelB with nanomolar range affinitye0

Not reportedMaterialCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

e0

“The bacterial plant pathogen Agrobacterium fabrum uses periplasmic-binding proteins (PBPs) along with ABC transporters to import a wide variety of plant molecules as nutrients. Nonetheless, how A. fabrum acquires plant metabolites is incompletely understood. Using genetic approaches and affinity measurements, we identified here the PBP MelB and its transporter as being responsible for the uptake of the raffinose family of oligosaccharides (RFO), which are the most widespread d-galactose–containing oligosaccharides in higher plants. We also found that the RFO precursor galactinol, recently described as a plant defense molecule, is imported into Agrobacterium via MelB with nanomolar range affinity. Structural analyses and binding mode comparisons of the X-ray structures of MelB in complex with raffinose, stachyose, galactinol, galactose, and melibiose (a raffinose degradation product) revealed how MelB recognizes the nonreducing end galactose common to all these ligands and that MelB has a strong preference for a two-unit sugar ligand. Of note, MelB conferred a competitive advantage to A. fabrum in colonizing the rhizosphere of tomato plants. Our integrative work highlights the structural and functional characteristics of melibiose and galactinol assimilation by A. fabrum, leading to a competitive advantage for these bacteria in the rhizosphere. We propose that the PBP MelB, which is highly conserved among both symbionts and pathogens from Rhizobiace family, is a major trait in these bacteria required for early steps of plant colonization. The bacterial plant pathogen Agrobacterium fabrum uses periplasmic-binding proteins (PBPs) along with ABC transporters to import a wide variety of plant molecules as nutrients.”