Claim · model-written summary
Hybrid assemblies combining both technologies yielded longer phytoplasma contigs than assemblies from Illumina datasets.
- Taxon
- phytoplasma
Candidatus Phytoplasmae0 - Outcome
- longer phytoplasma contigse0
- Direction
- longere0
- Comparator
- assemblies from Illumina datasetse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hybrid assemblies that combined both technologies, yielding longer phytoplasma contigs than assemblies from Illumina datasets”
Title
Kogej Zwitter, Zala; Kutnjak, Denis; Mehle, Nataša DiRROS repository (University of Maribor) 2025
Other claims from this paper (7)
- Method / resource The most effective protocol combined differential centrifugation, CTAB extraction and removal of CpG-methylated host DNA and notably increased the relative abundance of phytoplasma reads compared to the other protocols.
- Method / resource Rarefaction analysis showed that the entire phytoplasma genome was covered by reads in a dataset of 3 billion nucleotides generated with this protocol.
- Method / resource Illumina sequencing yielded more accurate assemblies with longer total lengths than nanopore sequencing.
- Method / resource Assemblies derived from nanopore sequencing data contained longer individual contigs than Illumina assemblies.
- Method / resource Hybrid assemblies had lower mismatch rates than assemblies from nanopore sequencing datasets.
- Method / resource A hybrid de novo assembled genome of the Slovenian Flavescence doree phytoplasma isolate achieved 96% reference genome coverage with high contiguity and low error rates.
- Method / resource The protocol enables high-quality genome sequencing of phytoplasma-infected grapevines without propagation in test plants.