Claim · model-written summary
Truncating the N-terminal signal peptide of IFN (mIFNγ) gave a nearly seven-fold increase in yield.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- Truncation of the N-terminal signal peptidee2
- Gene
- mIFNγe2
- Outcome
- yielde2
- Direction
- increasee2
- Value
- nearly seven-folde2
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e2“Truncation of the N-terminal signal peptide of IFN (designated mIFNγ) resulted in a nearly seven-fold increase in yield”
Title
Min-Chao Jiang; Chung-Chi Hu; Na-Sheng Lin; et al. Viruses 2019. doi:10.3390/v11060509
Other claims from this paper (5)
- Intervention effect The pKB△CHis BaMV vector gave the highest expression level of unmodified IFNγ among the modified vectors.
- Intervention effect Co-expressing the silencing suppressor P19 in place of the BaMV movement protein gave a 40% increase in mIFNγ accumulation.
- Intervention effect Fusing an ER retention signal to mIFNγ significantly enhanced the ratio of biologically active dimeric mIFNγ to 87%, relative to the non-active monomeric form.
- Intervention effect The pKB19mIFNγER construct, combining all enhancement strategies, gave the highest protein accumulation, up to 119 ± 0.8 μg/g fresh weight.
- Recommendation The authors advocate the modified BaMV-based vector as a platform for high-level expression of therapeutic protein in N. benthamiana.