Claim · model-written summary
SRS validated an AtNAP SAG113 promoter interaction.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“we demonstrated the feasibility of the SRS system in validating the interaction between the senescence-associated TF AtNAP (Zhang and Gan, 2012) and its downstream target gene, the SAG113 promoter”
t0“plant”
Title
Qi Zhou; Yulu Ye; Haiyan He; et al. Plant Biotechnology Journal 2024. doi:10.1111/pbi.14488
Other claims from this paper (19)
- Intervention effect Expression of all tested SGR1 genes accelerated yellowing in N. benthamiana leaves.
- Observation AtSGR1 caused the fastest and most significant chlorophyll degradation.
- Intervention effect Kozak sequence enhancement boosted chlorophyll degradation.
- Intervention effect Darkness boosted chlorophyll degradation.
- Intervention effect Maintaining 22 to 25°C boosted chlorophyll degradation.
- Method / resource Fourteen image features correlated with SPAD values at r greater than 0.5.
- Method / resource The stacking model predicted SPAD values with R2 of 0.85.
- Method / resource The stacking model predicted SPAD values with RMSE of 2.4.
- Method / resource The stacking model predicted SPAD values with NRMSE of 12.24%.
- Method / resource A homozygous CR19 plant had all six NbSGR1 genes edited.
- Observation CR19 delayed leaf yellowing in darkness.
- Observation CR19 reduced chlorophyll degradation in darkness.
- Method / resource The FAR1 FHY1 reporter turned leaves yellow while controls changed minimally.
- Observation SGR1 expression increased in the presence of FAR1 and the FHY1 promoter.
- Method / resource A mutated FAR1 and noninteracting OsTB1 promoter supported reporter specificity.
- Method / resource The TIG1 SAUR39 reporter caused significant colour changes and increased SGR1 transcript levels versus controls.
- Method / resource The MYB29 SUR1 construct produced expected colour changes.
- Method / resource avrBs3 and pepper Bs3 promoter coexpression produced noticeable colour changes.
- Method / resource SRS worked in rapeseed and lettuce, although some plants showed unexpected phenotypes.