Claim · model-written summary
Isolates 3-3-1 to 3-3-4 clustered monophyletically with N. clavispora in the concatenated ITS, TEF1-α and TUB2 phylogeny, with bootstrap support of 80%.
- Taxon
- E. pungens
Elaeagnus pungense1 - Interactor
- N. clavisporaNeopestalotiopsis clavisporae7pathogen
- Outcome
- clustered monophyleticallye7
- Value
- 80%e7
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a leaf spot disease was found on E. pungens in the campus of Nanjing Forestry University, China”
e7“Phylogenetic analysis using concatenated sequences of ITS, TEF1-α, and TUB2 also showed that isolate 3-3-1, 3-3-2, 3-3-3, and 3-3-4 clustered monophyletically with N. clavispora, and supported with a high bootstrap value (80%)”
Title
First Report of Leaf Spot on Elaeagnus pungens Caused by Neopestalotiopsis clavispora in China
Xi-Ling Qi; Jiao He; Yun-Wei Ju; et al. Plant Disease 2022. doi:10.1094/pdis-10-22-2457-pdn
Other claims from this paper (9)
- Observation Leaf spot on E. pungens in a campus of Nanjing Forestry University had an incidence rate of ca. 77%.
- Observation Leaf spot on E. pungens began as small brown spots on the leaves that enlarged and coalesced into gray necrotic lesions with dark margins.
- Observation The ITS, TEF1-α and TUB2 sequences of the isolates showed 100%, 97% and 99% similarity to Neopestalotiopsis clavispora.
- Observation Isolates 3-3-1 to 3-3-4 were identified as N. clavispora based on phylogeny and morphology.
- Observation This is the first report of leaf spot caused by N. clavispora on E. pungens worldwide.
- Observation Isolate 3-3-4 grew at an average of 12.2 mm per day on PDA at 25 °C.
- Observation Conidia of the isolates were spindle shaped with five cells and four septa, averaging 23.31 ± 1.81 × 7.33 ± 1.07 μm.
- Observation The pathogen was isolated from diseased E. pungens tissue at a frequency of ca. 100%.
- Intervention effect Inoculation of E. pungens leaves with mycelial plugs or conidial suspensions of isolate 3-3-4 produced necrotic lesions, whereas PDA discs and dH2O produced none.