Claim · model-written summary
Tomato root samples had higher absorbance than ooze and soil.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- roote0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Outcome
- absorbancee0
- Direction
- highe0
- Comparator
- ooze and soile0
Not reportedMaterialGeneCompoundSecond interactorSecond interactor roleTreatmentSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sensitivityof detectionof R. solanacearum in different samples has indicated that high absorbance in root samples followed by ooze and soil in all the hosts tested such as chilli, brinjal and tomato.”
Title
Immunodiagnosis of bacterial wilt pathogen using polyclonal antiserum to Ralstonia solanacearum
M Manasa; M. Krishnareddy; C. Gopalakrishnan; et al. Pest Management in Horticultural Ecosystems 2015
Other claims from this paper (12)
- Method / resource A DAC immune assay was developed to detect virulent Ralstonia solanacearum in plants and soil.
- Mechanism EPS was identified as a major virulence factor.
- Intervention effect Antigen type significantly influenced antibody specificity, with whole killed cells producing higher specificity than extracellular polysaccharides.
- Observation EPS may be a weaker immunogen than whole cells.
- Method / resource Both antisera detected Ralstonia natural infection in tomato at 1:8000 dilution.
- Method / resource Both antisera detected Ralstonia natural infection in brinjal at 1:8000 dilution.
- Method / resource Both antisera detected Ralstonia natural infection in chilli at 1:8000 dilution.
- Method / resource Both antisera detected bacterial cultures and infected soil at 1:8000 dilution.
- Observation Chilli root samples had higher absorbance than ooze and soil.
- Observation Brinjal root samples had higher absorbance than ooze and soil.
- Method / resource Pure culture had a higher detection limit, up to 1012 cells per ml, than plant and soil samples.
- Method / resource The assay is semiquantitative and proportional to pathogen concentration.