Method / resourcemodel-written summary

Specificity, selectivity and repeatability remained unchanged in multiplex versus singleplex RT-qPCR.

A claim from Optimization and Validation of Singleplex and Multiplex RT-qPCR for Detection of Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), and Hop stunt viroid (HSVd) in Hops (Humulus lupulus) · 2023

Method / resourceobservedoriginal resultno effectmeasured

Claim · model-written summary

Specificity, selectivity and repeatability remained unchanged in multiplex versus singleplex RT-qPCR.

Taxon
Hopst0
Outcome
specificity, selectivity, repeatabilitye0
Direction
remained unchangede0
Comparator
singleplex RT-qPCRe0

Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingValueQualifier

Verbatim

e0

“Method validation showed that mRT-qPCR had lower sensitivity than singleplex RT-qPCR, while specificity, selectivity, repeatability, and reproducibility remained unchanged.”

✓ located verbatim in the source · abstract, characters 1204–1376
t0

“Hops (Humulus lupulus)”

✓ located verbatim in the source · title, characters 175–197

Title

Optimization and Validation of Singleplex and Multiplex RT-qPCR for Detection of Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), and Hop stunt viroid (HSVd) in Hops (Humulus lupulus)

Tanja Guček; Jernej Jakše; Sebastjan Radišek Plant Disease 2023. doi:10.1094/pdis-11-22-2606-re

Other claims from this paper (3)

Record rev-628dbf854f94fdf8aaa1aa35ae3afafe718b2e1d7033892edc254e518d545f35 · schema claims-v0.9 · position 2 in the model’s answer