Claim · model-written summary
A modified purine procedure yielded three to four times more purines than the original procedure in duodenal samples and NDF residue.
- Taxon
- foraget0
- Outcome
- purinese1
- Direction
- moree1
- Value
- three to four times moree1
- Comparator
- original purine proceduree0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingQualifier
Verbatim
t0“Estimating forage protein degradation in the rumen”
e0“Recent reports suggested that the original purine procedure generates interfering compounds that reduce estimates of microbial protein”
e1“Reanalysis of samples with a modified purine procedure yielded three to four times more purines in both duodenal samples and NDF residue incubated in situ”
Title
Estimating forage protein degradation in the rumen
T. J. Klopfenstein; R. A. Mass; K. W. Creighton; et al. Journal of Animal Science 2001. doi:10.2527/jas2001.79e-supple208x
Other claims from this paper (9)
- Method / resource The alternative in situ NDIN procedure correlates highly with the purine-corrected in situ procedure.
- Method / resource The alternative in situ procedure is less variable than the purine-corrected in situ procedure and simpler to perform.
- Recommendation Differential centrifugation may be inappropriate for obtaining clean samples of rumen microbes.
- Recommendation The authors suggest measuring the purine-to-N ratio with NDF incubated in situ and particle-associated microorganisms measured.
- Recommendation The authors propose adding a lag time associated with passage to the UIP calculation.
- Intervention effect Degradation with no passage (applying a lag) reduced UIP values of forage with high potentially available UIP pools.
- Method / resource Enzyme analysis and near infrared reflectance spectroscopy show promise for measuring UIP when fistulated cattle are unavailable.
- Recommendation The in situ NDIN procedure appears to be a simple and acceptable method of UIP determination.
- Recommendation The modified purine assay should be considered when purine measurement is needed.