Claim · model-written summary
The entire workflow, from simplified DNA extraction to visual interpretation, can be completed within 40 min.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Interactor
- Alternaria alternata
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Alternaria alternatat0pathogen - Outcome
- entire workflowe1
- Value
- within 40 mine1
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
.jpg?width=500)
Verbatim
t0“Alternaria alternata causing pear black spot”
e1“The entire workflow, from simplified DNA extraction to visual interpretation of results, can be completed within 40 min”
Title
Liqing Zhang; Wei Jiang; Zhiwei Song; et al. LWT 2026. doi:10.1016/j.lwt.2026.119301
Other claims from this paper (3)
- Method / resource The RPA-CRISPR/Cas12a assay achieved a detection limit of 1 copy/μL per reaction for Alternaria alternata, surpassing the sensitivity of conventional PCR/qPCR.
- Method / resource The optimized assay showed high specificity for A. alternata.
- Method / resource Field validation with 54 pear leaf and fruit samples showed complete concordance with PCR.