Claim · model-written summary
The efficiency of mature somatic embryo maturation varied greatly with the basal medium and ABA concentration.
- Taxon
- Picea pungens
Picea pungenst0 - Treatment
- concentration of abscisic acid (ABA)e1
- Outcome
- efficiencye1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the efficiency, expressed as the number of mature somatic embryos per gram of fresh mass cultured (E/gFM), varied greatly with the choices of the basal medium and concentration of abscisic acid (ABA) of the maturation medium”
t0“Picea pungens”
Title
Xi Cao; Fang Gao; Caiyun Qin; et al. Forests 2022. doi:10.3390/f13122097
Other claims from this paper (13)
- Intervention effect The optimum initiation concentration of 2,4-D was 4.0 mg/L for maximizing initiation efficiency.
- Intervention effect The optimum initiation concentration of 6-BA was 2 mg/L for maximizing initiation efficiency.
- Intervention effect The optimum initiation sucrose concentration was 5 to 10 g/L for maximizing initiation efficiency.
- Recommendation Using the DCR medium as the basal medium and 10 mg/L ABA was the judicious choice for maturation.
- Intervention effect Adjusting the maturation medium osmotic pressure improved maturation efficiency.
- Intervention effect Using sucrose as the carbohydrate source facilitated maturation.
- Intervention effect A low (<8 g/L) Gelrite concentration in the maturation medium facilitated maturation.
- Observation Optimal maturation selections were genotype-dependent.
- Recommendation Culturing more than 100 mg embryogenesis tissue per dish is needless.
- Recommendation The optimum pretreatment for embryogenesis tissue cryopreservation was proliferation medium with 0.4 mol/L sorbitol for 24 h, followed by 5% DMSO.
- Observation The study significantly improved somatic embryo initiation to a frequency of 0.56.
- Observation The study significantly improved embryo maturation efficiency to 1030 E/gFM.
- Observation The preculturing protocol recovered 1354 E/gFM after cryopreservation.