Claim · model-written summary
In situ hybridization staining patterns in plantlets from somatic embryos were similar to the signals in seedlings grown from seeds.
- Taxon
- Norway spruce
Picea abiest0 - Organ / stage
- plantlets derived from somatic embryose1
- Outcome
- staining patternse1
- Comparator
- staining signals seen in seedlings grown from seedse1
Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Aquaporin homologues in Norway spruce (Picea abies)”
e1“The in situ hybridization staining patterns in plantlets derived from somatic embryos were similar to the staining signals seen in seedlings grown from seeds”
Title
Aquaporin homologues in Norway spruce (Picea abies)
Peter Oliviusson 1999
Other claims from this paper (16)
- Observation Immunoblot analyses indicated at least two aquaporin isoforms in Norway spruce seeds during development.
- Observation One aquaporin isoform was abundant in mature Norway spruce seeds.
- Observation Another aquaporin isoform was detected in extracts of young Norway spruce seedlings, particularly in the roots.
- Observation Full-length gene mipfg was identified from Norway spruce seeds.
- Observation Full-length gene mipr was identified from Norway spruce roots.
- Observation The two identified genes showed high homology to the tonoplast sub-class of aquaporins.
- Observation In mature seeds, aquaporin labelling was high in the tonoplast of protein storage vesicles.
- Observation Before protein storage vesicle formation, labelling was seen in the plasmamembrane of jacket cells lining the archegonia in young female gametophytes.
- Observation In situ hybridization gave strong expression signals in jacket cells and in maturing seeds of Norway spruce.
- Observation In maturing seeds, the hybridization signal was found in the entire female gametophyte.
- Observation A strong signal was seen in the spongy tissue surrounding the female gametophyte of early ovules.
- Observation The in situ high signal corresponded well with results from Northern analyses.
- Observation A strong hybridization signal was seen in root tips of plants of various ages, including lateral roots and adventitious roots.
- Observation Columella cells of the root cap and guard cells of stomata in needles stained intensely.
- Observation A gene transcript identical to mipfg was identified in somatic embryos of Norway spruce.
- Mechanism Aquaporin function is indicated to have important roles in regulating transmembrane water and solute transport in Norway spruce.