Claim · model-written summary
B-1015 was confirmed as a stable single T-DNA insertional mutant.
- Taxon
- rice
Oryza sativae0 - Interactor
- B-1015e1pathogen
- Outcome
- stable single T-DNA insertional mutante1
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogenicity in rice”
e1“the B-1015 was a stable single T-DNA insertional mutant”
Title
Huang Le Zhongguo nongye Kexue 2014
Other claims from this paper (12)
- Observation B-1015 sporulation and growth rate declined significantly versus wild type P1.
- Observation B-1015 spore germination rate did not differ significantly from P1.
- Observation The insertional mutant lost pathogenicity in rice.
- Observation T-DNA flanking sequences were obtained by hiTail-PCR.
- Observation Uvt-1015R and Uvt-1015L were cloned by RACE PCR.
- Observation Uvt-1015R had a 948 bp open reading frame encoding a 295 amino acid protein and 341 bp and 271 bp untranslated regions.
- Observation Uvt-1015L had a 351 bp open reading frame coding a 116 amino acid protein and 31 bp and 174 bp untranslated regions.
- Observation Neither Uvt-1015R nor Uvt-1015L had homology to a known protein.
- Observation T-DNA flanking sequences were nonadjacent in the wild type genome, and T-DNA disrupted two gene sequences.
- Observation Uvt-1015R transcripts decreased significantly compared with P1.
- Observation Uvt-1015L transcripts were no longer detected.
- Mechanism The authors attributed B-1015 loss of pathogenicity to T-DNA insertion and chromosomal rearrangement.