Claim · model-written summary
The VdEGe1 knockout mutant of V. dahliae showed impaired colony expansion and hyphal growth.
- Taxon
- Verticillium dahliae
Verticillium dahliaet0 - Treatment
- ΔVdEGe1 knockoute1
- Gene
- VdEGe1e1
- Outcome
- colony expansion, hyphal growthe1
- Direction
- impairede1
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The ΔVdEGe1 knockout mutant exhibited impaired colony expansion, hyphal growth, sporulation, and spore germination, accompanied by severely attenuated virulence”
t0“Verticillium dahliae”
Title
VdEGe1, a cell wall-degrading enzyme gene from Verticillium dahliae required for cotton pathogenesis
Yuanjing Li; Ruixiang Yuan; Yongtai Li; et al. BMC Microbiology 2026. doi:10.1186/s12866-026-04977-x
Other claims from this paper (11)
- Observation VdEGe1 of Verticillium dahliae was classified into glycoside hydrolase family 45 (GH45).
- Intervention effect Host-induced gene silencing of VdEGe1 significantly reduced Verticillium wilt symptoms, disease index and fungal biomass in infected cotton.
- Intervention effect The VdEGe1 knockout mutant of V. dahliae showed impaired sporulation and spore germination.
- Intervention effect The VdEGe1 knockout mutant of V. dahliae had severely attenuated virulence.
- Observation VdEGe1 was confirmed to be a secreted protein.
- Intervention effect VdEGe1 did not trigger or suppress cell death in Nicotiana benthamiana.
- Observation Secretome analysis identified 311 differentially expressed secretory proteins.
- Observation Of the differentially expressed secretory proteins, 156 were down-regulated.
- Intervention effect The VdEGe1 deletion mutant showed significant down-regulation of mitochondrial-related proteins and several carbohydrate-active enzymes.
- Intervention effect The VdEGe1 deletion mutant had decreased intracellular ATP levels and slower growth on media with various carbon sources.
- Mechanism The authors conclude that VdEGe1 deletion leads to mitochondrial dysfunction and impaired carbon utilization, inducing energy starvation in V. dahliae and compromising its growth, development and virulence.