Claim · model-written summary
Copper increased ascorbate oxidase protein abundance.
- Taxon
- Pumpkint0
- Treatment
- adding coppere0
- Gene
- ascorbate oxidase proteine0
- Outcome
- amount of ascorbate oxidase proteine0
- Direction
- increasede0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Verbatim
e0“Furthermore, immunological blotting showed that the amount of ascorbate oxidase protein was also increased by adding copper.”
t0“Pumpkin”
Title
Regulation of Ascorbate Oxidase Expression in Pumpkin by Auxin and Copper
Muneharu Esaka; Kouichi Fujisawa; Miwa Goto; et al. PLANT PHYSIOLOGY 1992. doi:10.1104/pp.100.1.231
Other claims from this paper (16)
- Observation Leaf ascorbate oxidase activity was about twice fruit activity.
- Observation Stem ascorbate oxidase activity was about 1.5 times fruit activity.
- Observation Seeds had little ascorbate oxidase activity.
- Observation Ascorbate oxidase mRNA was abundant in pumpkin leaves and stems.
- Observation Fruit ascorbate oxidase mRNA was lower than in leaves and stems.
- Observation Ascorbate oxidase mRNA was undetected in pumpkin seeds.
- Observation Specific ascorbate oxidase activity rose during early seedling growth.
- Observation Ascorbate oxidase mRNA also increased during early seedling growth.
- Observation Immature rapidly growing pumpkin fruits had much higher ascorbate oxidase activity than mature fruits.
- Intervention effect 2,4-D increased ascorbate oxidase activity in cultured pumpkin fruit tissue.
- Intervention effect 2,4-D increased ascorbate oxidase mRNA in cultured pumpkin fruit tissue.
- Intervention effect Ascorbate oxidase mRNA was most abundant at 10 mg/L 2,4-D.
- Mechanism The authors infer that ascorbate oxidase participates in cell growth.
- Intervention effect Copper increased ascorbate oxidase activity in pumpkin callus.
- Intervention effect Copper did not increase ascorbate oxidase mRNA.
- Mechanism Copper may regulate ascorbate oxidase at translation or a later step.