Claim · model-written summary
Mechanical inoculation and agroinfiltration failed to establish PapDfV1 infection in common bean.
- Taxon
- common bean
Phaseolus vulgarise0 - Treatment
- Mechanical inoculation and agroinfiltratione0
- Interactor
- PapDfV1e0other
- Outcome
- infect PapDfV1e0
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mechanical inoculation and agroinfiltration failed to infect PapDfV1 in papaya, Nicotiana benthamiana, and common bean”
Title
Milenka Vera; Raúl Cifuentes; Ronan Keener; et al. PLoS ONE 2025. doi:10.1371/journal.pone.0329708
Other claims from this paper (15)
- Observation A novel carlavirus-like RNA named PapDfV1 was identified in papaya latex RNA.
- Observation PapDfV1 has two open reading frames.
- Observation PapDfV1 ORF 1 encodes a 211.1 kDa replicase.
- Observation PapDfV1 replicase shares 96% sequence identity with ZhBV2.
- Observation PapDfV1 ORF 2 is chimeric with regions homologous to two ZhBV2 ORFs.
- Observation PapDfV1 has a 2.1 kb deletion spanning portions of TGB1 and NABP and all of TGB2, TGB3 and CP.
- Observation Replicase phylogeny grouped PapDfV1 with ZhBV2 and three Chinese CPMMV isolates.
- Observation Two Chinese CPMMV isolates share the reported terminal sequences.
- Intervention effect Mechanical inoculation and agroinfiltration failed to establish PapDfV1 infection in papaya.
- Intervention effect Mechanical inoculation and agroinfiltration failed to establish PapDfV1 infection in Nicotiana benthamiana.
- Intervention effect A ZhBV2 construct successfully infected soybean.
- Intervention effect A ZhBV2 construct successfully infected common bean.
- Intervention effect A ZhBV2 construct successfully infected N. occidentalis.
- Observation PapDfV1 was detected in total and double-stranded RNA from leaves of a two-year-old papaya.
- Mechanism PapDfV1 replication and movement may depend on host proteins in mature papaya or unknown co-infecting viruses.