Claim · model-written summary
rRNA depleted sequencing recovered longer viral contigs.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValueQualifier
Verbatim
e0“rRNA-depleted total RNA-seq consistently recovered more viruses, longer contigs, and complete multipartite DNA virus genomes”
e1“pepper leaves (Anseong and Jincheon) and garlic cloves (Hoengseong)”
Title
Hoseong Choi; Dong Woo Kang; Yeonhwa Jo; et al. International Journal of Molecular Sciences 2026. doi:10.3390/ijms27052300
Other claims from this paper (9)
- Method / resource rRNA depleted sequencing recovered more viruses than mRNA sequencing.
- Method / resource rRNA depleted sequencing recovered complete multipartite DNA virus genomes.
- Method / resource mRNA sequencing was dominated by highly expressed polyadenylated viruses.
- Method / resource In Jincheon pepper, mRNA sequencing missed hot pepper endornavirus.
- Method / resource In Jincheon pepper, mRNA sequencing missed pepper cryptic virus 2.
- Method / resource In Jincheon pepper, mRNA sequencing missed several milk vetch dwarf virus segments.
- Method / resource Total RNA sequencing also detected low titer RNA viruses in garlic libraries, possibly contamination.
- Recommendation The authors recommend rRNA depleted total RNA sequencing for comprehensive virus discovery and genome reconstruction.
- Method / resource mRNA sequencing remains useful for polyadenylated virus quantification and host gene expression analysis.