Claim · model-written summary
FISH and karyotyping confirmed random integration of the V2 RNAi amplicon at chromosomes 6 and 16.
- Taxon
- Cotton
Gossypium hirsutumt0 - Gene
- V2 RNAi amplicone0
- Outcome
- random integration of V2 RNAi amplicon at chromosome 6 and 16e0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Copy number and transgene location were observed using FISH and karyotyping in T 2 generation which confirmed random integration of V2 RNAi amplicon at chromosome 6 and 16.”
t0“Cotton Plants”
Title
Aneela Yasmeen; Sarfraz Kiani; Afshan Butt; et al. Molecular Biotechnology 2016. doi:10.1007/s12033-016-9980-8
Other claims from this paper (5)
- Observation Transformation efficiency was 3.75% in MNH-786.
- Observation Transformation efficiency was 2.88% in VH-289.
- Observation PCR confirmed successful transformation in three generations.
- Intervention effect Promising transgenic cotton lines had low virus titer compared with wild type controls after whitefly challenge.
- Recommendation The authors concluded that the V2 RNAi construct limited virus replication and could control CLCuV in the field.