A two-step digestion protocol with different enzymatic buffers produced viable Chirita pumila protoplast suspensions for expression analysis and scRNA-seq

Claim · model-written summary

A two-step digestion protocol with different enzymatic buffers produced viable Chirita pumila protoplast suspensions for expression analysis and scRNA-seq

Taxon

Chirita pumilaHenckelia pumilae0

Treatment

two consecutive digestion processes with different enzymatic bufferse2

Outcome

viable cell suspensionse1

Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier

Verbatim

e0

“we describe the establishment of a simple and efficient protocol for protoplast preparation in Chirita pumila”

e1

“Using this protocol, we generated viable cell suspensions suitable for an array of expression analyses, including scRNA-seq”

e2

“which includes two consecutive digestion processes with different enzymatic buffers”