Claim · model-written summary
Pm41b carries two DNA transposons inserted in its promoter and 3′-UTR regions.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- Pm41be1
- Outcome
- two DNA transposonse1
- Qualifier
- inserted in the promoter and 3′-UTR regionse1
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Provoking a silent R gene in wheat genome confers resistance to powdery mildew”
e1“Pm41b contains an intact Pm41a allelic coding region sequence (Figure S1) but carries two DNA transposons inserted in the promoter and 3′-UTR regions (Figures 1c and S2)”
Title
Provoking a silent R gene in wheat genome confers resistance to powdery mildew
Miaomiao Li; Lei Dong; Lei Dong; et al. Plant Biotechnology Journal 2022. doi:10.1111/pbi.13903
Other claims from this paper (19)
- Observation Seven Pm41 haplotypes were identified in the tested wheat panel.
- Observation Four new Pm41 haplotypes (Hap4 to Hap7) were identified in WEW populations.
- Observation Only three Pm41 haplotypes (Hap2, Hap3, and Hap7) occur in durum and hexaploid wheat populations.
- Mechanism Loss of genetic diversity at the Pm41 locus is suggested to result from domestication and polyploidization bottleneck.
- Observation Hap2 (Pm41b) accounts for 5% of the WEW accessions.
- Observation Hap2 (Pm41b) is predominant in the tested durum accessions, at 90%.
- Observation Hap2 (Pm41b) is predominant in the tested hexaploid wheat accessions, at 90%.
- Observation Pm41b contains an intact Pm41a allelic coding region sequence.
- Observation The native Pm41a allele is resistant and well induced after Bgt isolate E09 inoculation in the resistant wild emmer accession IW2.
- Intervention effect Pm41b shows no expression before or after Bgt isolate E09 inoculation in the susceptible durum cultivar LDN.
- Observation A similar lack of Pm41b expression is found in the susceptible common wheat cultivar Fielder, which carries the same Pm41b allele as LDN.
- Mechanism Pm41b and other Pm41 haplotypes are suggested to be silenced alleles of Pm41a, probably due to transposon insertions.
- Intervention effect Transgenic plants carrying the ubiquitin-driven Pm41b gene were immune to Bgt isolate E09.
- Intervention effect Transgenic plants carrying Pm41b under the native Pm41a promoter were resistant to isolate E09, with IT of 1 (highly resistant).
- Observation The Pm41b transgene co-segregates with powdery mildew resistance in T2 segregating progenies.
- Intervention effect The Pm41b transcript level in ubiquitin-driven transgenic plants is about three-fold higher than in plants with the native Pm41a promoter.
- Mechanism Pm41b is suggested to function in a dosage-dependent way.
- Intervention effect Increased powdery mildew resistance of Pm41b-OE and Pm41-COM transgenic plants did not affect major agronomic traits.
- Recommendation Silent loci like Pm41b are suggested to be a valuable resource of genetic variation that could be used to enrich diversity in wheat breeding.