Claim · model-written summary
A low molecular weight RNA–protein complex was released from rye ribosomes after formamide treatment.
- Taxon
- Rye embryot0
- Treatment
- formamidee1
- Outcome
- A low molecular weight complex of RNA and protein (RNP)e0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Verbatim
e0“A low molecular weight complex of RNA and protein (RNP) was released.”
t0“rye embryos”
e1“the large subunit fraction was treated with formamide.”
Title
Analysis of a 5S RNA–protein complex isolated from the ribosomes of rye embryos
Tristan A. Dyer; Saul Zalik Canadian Journal of Biochemistry 1979. doi:10.1139/o79-186
Other claims from this paper (5)
- Observation Electrophoresis of the rye RNP yielded one RNA band and one protein band.
- Observation The rye RNP protein had an approximate molecular weight of 41 000.
- Observation The rye RNP RNA component was identified as 5S ribosomal RNA.
- Observation Protein shielded the entire 5S RNA to some extent, with greatest protection at nucleotides 68 to 108.
- Mechanism The authors infer that nucleotides 68 to 108 primarily interact with protein in the complex and possibly in ribosomes.