Claim · model-written summary
The TBSV chimera carrying CNV coat protein was transmitted to cucumber seedling roots by fungal zoospores.
- Taxon
- cucumber
Cucumis sativuse0 - Interactor
- TBSVTomato bushy stunt viruse0pathogen
- Second interactor
- O. radicalee0other
- Gene
- CNV coat proteine0
- Outcome
- were transmittede0
Not reportedMaterialCompoundOrgan / stageTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transmission to roots of cucumber seedlings by zoospores of a cucumber strain of O. radicale. Particles produced by the TBSV chimeric clone encoding the CNV coat protein were transmitted”
Title
Molecular characterization of the cucumber necrosis virus coat protein gene
McLean, Morven A. cIRcle (University of British Columbia) 2008. doi:10.14288/1.0098844
Other claims from this paper (15)
- Intervention effect PD(-) transcripts produced significantly smaller local lesions than wild type in Nicotiana clevelandii.
- Intervention effect Systemic symptoms after PD(-) transcript inoculation developed 8 to 12 days later than after wild type inoculation.
- Observation Sap from PD(-) transcript infected plants yielded wild-type sized lesions and reduced or lost delay of systemic symptoms in N. clevelandii.
- Observation PD(-) transcript and sap inoculation produced either wild-type systemic necrosis or oak-leaf necrosis in N. clevelandii.
- Observation Full-length PD(-) RNA was only rarely detected in transcript-inoculated leaves.
- Observation A stable further deleted RNA species of approximately 3800 nucleotides occurred in systemically infected leaves.
- Observation CNV coat protein was undetectable in PD(-) infected leaves.
- Observation Sequenced 3.8 kb RNA retained only 74 of 1140 nucleotides of the coat protein open reading frame.
- Observation Coat-proteinless CNV transcripts produced wild-type and oak-leaf necrosis in N. clevelandii, resembling PD(-) sap inoculation.
- Intervention effect Coat-proteinless CNV tended to delay systemic symptoms compared with wild type.
- Mechanism CNV replicated and moved systemically in inoculated N. clevelandii without functional coat protein.
- Intervention effect Mechanical transmission of CNV without coat protein was less efficient than wild type.
- Observation Both reciprocal CNV and TBSV coat protein chimeras infected N. clevelandii.
- Observation The CNV chimera carrying TBSV coat protein was not transmitted by fungal zoospores to cucumber seedling roots.
- Mechanism The CNV coat protein gene specifically determines fungal transmissibility.