Claim · model-written summary
The reaction demonstrated complete replication of TMV-L RNA, with a positive-strand product uniformly labelled along its length.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- tobacco mosaic tobamoviruse0pathogen
- Outcome
- complete replication of TMV-L RNAe6
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extracts of tomato leaves infected with tobacco mosaic tobamovirus (tomato strain L) TMV-L)”
e6“This was shown to be the positive strand and uniformly labelled along its length, demonstrating complete replication of TMV-L RNA”
Title
T A Osman; K W Buck Journal of Virology 1996. doi:10.1128/jvi.70.9.6227-6234.1996
Other claims from this paper (10)
- Intervention effect Removal of endogenous RNA with micrococcal nuclease made the tomato-leaf membrane polymerase template dependent and template specific.
- Observation The membrane polymerase was primer independent and initiated RNA synthesis on TMV-L 3'-terminal positive or negative strand templates.
- Intervention effect TMV-vulgare RNA was a less efficient template for the polymerase.
- Intervention effect RNAs of cucumber mosaic cucumovirus and red clover necrotic mosaic dianthovirus did not act as templates for the polymerase.
- Observation A main product of the reaction with TMV-L genomic RNA as template was genomic-length single-stranded RNA.
- Observation Genomic-length double-stranded RNA labelled in both strands was formed, with small amounts of subgenomic RNA forms.
- Observation Antibodies to the 126-kDa protein detected the 126-kDa and 183-kDa readthrough proteins in purified polymerase preparations.
- Observation Antibodies to the readthrough portion of the 183-kDa protein detected only the 183-kDa protein.
- Intervention effect All three antibodies inhibited the template-dependent RNA polymerase.
- Intervention effect None of the three antibodies had any effect on the template-bound polymerase.