Claim · model-written summary
Geneticin sulfate produced 4.8 times significantly more transgenic sugarcane plants than paromomycin sulfate after direct embryogenesis.
- Taxon
- sugarcanee0
- Treatment
- geneticin sulfatee5
- Outcome
- transgenic plantse5
- Direction
- significantly moree5
- Value
- 4.8 timese5
- Comparator
- paromomycin sulfatee5
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingQualifier
Verbatim
e0“The comparison of direct and indirect somatic embryogenesis (DSE and ISE) for biolistic transformation of sugarcane”
e5“When geneticin sulfate, was used for selection following DSE, significantly more (4.8 times) transgenic plants were produced than with paromomycin sulfate”
Title
Yogesh TapariaMaria GalloFredy Altpeter 2012
Other claims from this paper (6)
- Method / resource Indirect somatic embryogenesis gave the highest transformation efficiency in sugarcane, at 2.2 independent transgenic plants per shot, compared with direct embryogenesis.
- Method / resource Direct somatic embryogenesis gave the most rapid production of transgenic sugarcane plants, within 12 weeks from explant to plants in soil.
- Method / resource Microprojectiles of 0.3 µm diameter produced 5 times more transgenic lines in sugarcane than 1 µm microprojectiles.
- Intervention effect Significantly fewer hybridization signals in Southern blots were observed with 0.3 µm microprojectiles than with 1.0 µm microprojectiles in sugarcane.
- Method / resource The number of non-expressing sugarcane plants was equal under both selection agents.
- Method / resource Optimized protocols generated rooted transgenic plants of a sugarcane cultivar with simple transgene integration within 12 or 19 weeks of culture initiation.