Claim · model-written summary
The protocol produced 20 transgenic clones per 0.5g inoculated tissue with 80% embryo survival in Kent mango.
- Taxon
- mango
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Mangifera indicat0 - Material
- Kente1
- Outcome
- transgenic clonese0
- Value
- Twenty transgenic clones per 0.5ge0
- Qualifier
- 80% embryo survivale0
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Twenty transgenic clones per 0.5g of inoculated tissues with 80% embryo survival were produced”
e1“transgenic somatic embryos and plants of the Kent variety”
t0“mango”
Title
Insertion of Agrobacterium rhizogenes rolB Gene in mango
Marleny Chavarri; Ariadne Vegas García; Asia Y. Zambrano; et al. Redalyc (Universidad Autónoma del Estado de México) 2010
Other claims from this paper (5)
- Observation The Kent variety required 20 cefotaxime/carbencillin washes to eliminate A. rhizogenes in the first treatment.
- Observation One to five washes eliminated A. rhizogenes in the other treatments.
- Intervention effect Acetosyringone preculture did not produce differences between treatments.
- Observation Infected embryos formed callus and secondary embryos before plant regeneration, without inducing hairy roots.
- Observation PCR confirmed the rolB gene in transformed mango leaf tissue, with an expected 720bp band only in transformed plants.