Claim · model-written summary
Only one nucleotide change was found in the barley genome at the highest gamma-ray dose
- Taxon
- barley
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Hordeum vulgaret0 - Treatment
- gamma rayse0
- Outcome
- nucleotide changee0
- Value
- onee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Only one nucleotide change was found after an analysis of 3,207,444bp derived from the highest dose of gamma rays applied”
t0“barley genome”
Title
Molecular analysis of point mutations in a barley genome exposed to MNU and gamma rays
Marzena Kurowska; Anna Labocha-Pawłowska; Dominika Gnizda; et al. Mutation research. Fundamental and molecular mechanisms of mutagenesis 2012. doi:10.1016/j.mrfmmm.2012.08.008
Other claims from this paper (11)
- Observation MNU induced an average mutation density of 1/504kb in the barley genome by TILLING
- Intervention effect MNU was a more efficient mutagen than gamma rays in inducing point mutations in barley
- Observation Transitions made up 63.6% of MNU-induced nucleotide changes in barley
- Observation G>A and C>T substitutions occurred in a similar number among MNU-induced changes in barley
- Mechanism The similar share of G>A and C>T transitions indicates no strand bias in the repair of O(6)-methylguanine lesions in barley
- Observation Purines formed 81% of nucleotides observed at the -1 site next to the O(6)-methylguanine lesion in barley
- Observation A strong specificity of the nucleotide surrounding the O(6)-meG lesion at the -1 position was observed in barley
- Intervention effect MNU-treated barley showed about three times higher AFLP polymorphism than the gamma-irradiated population
- Observation About 94% of AFLP bands in barley were heterogenic
- Observation Some AFLP bands in barley contained up to 8 different amplicons
- Observation The sequence types in polymorphic AFLP bands reflected the organisation of the barley genome