Claim · model-written summary
Manipulating culture duration and dilution ratios at subculture yielded suspensions consisting predominantly of embryogenic cells.
- Taxon
- Pennisetum americanum
Cenchrus americanust0 - Treatment
- manipulating the duration of culture and dilution ratiose2
- Outcome
- suspensions consisting predominantly of embryogenic cellse2
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“DERIVED FROM CULTURED INFLORESCENCES OF PENNISETUM AMERICANUM”
e2“By manipulating the duration of culture and dilution ratios (cell suspension: fresh medium) at the time of subculture, suspensions consisting predominantly of embryogenic cells were obtained”
Title
Vimla Vasil; Indra K. Vasil American Journal of Botany 1982. doi:10.1002/j.1537-2197.1982.tb13392.x
Other claims from this paper (7)
- Observation An embryogenic suspension culture was established from cultured inflorescence segments of Pennisetum americanum.
- Observation The suspension was composed of two major cell types.
- Intervention effect Transfer to agar media with reduced amounts of 2,4-D produced hundreds of globular and early cotyledonary embryoids.
- Intervention effect A medium containing abscisic acid promoted the development of the embryoids.
- Observation Many embryoids germinated and produced normal green plants.
- Observation Atypical embryoids containing many shoot meristems and a leafy scutellum were observed.
- Observation Somatic embryogenesis is suggested to occur in tissue cultures of most, if not all, cereal and grass species.