Abstract
The present experiment was conducted to study the effects of different explant treatment methods,hormone combinations and sugar concentrations on the induction,subculture and rooting in adventitious bud derived from embryonic leaf of peanut and to develop a regeneration system for highly efficient genetic transformation studies in peanuts.The main cultivated varieties of Guangxi,viz.,Guihua 17 and Guihua 22 were used and their seeds were precultured for 0,4,8 and 12 days on MS medium.The results showed that the embryonic leaf derived from seeds precultured for 0 and 4 days were suitable for the induction of adventitious bud for higher differentiation rate.The differentiation rate of adventitious bud derived from 1 / 3 cross-section of embryonic leaves near petiole was higher than of the portion far away from petiole.It was found that 40 and 50g / L sugar in MS medium was best suited for the induction of adventitious bud derived from embryonic leaves of Guihua 22 and Guihua 17,respectively.The medium MS + 4 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 and MS + 6 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 was beneficial for the differentiation of adventitious buds in Guihua 17 and Guihua 22,respectively,with 100% of differentiation rate.The subculture medium MS + 1.5 mg / L 6-BA + 0.4 mg / L NAA was suitable for promoting the growth of adventitious buds and development of plantlets,and the best medium for rooting of plantlet was 1 / 2 MS + 0.5 mg / L 6-BA + 2.0 mg / L NAA.The findings of the experiment can be used for the genetic transformation in peanuts with higher differentiation rate of adventitious bud and regeneration of plantlets.
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Claims 8
Claim · model-written summary
Seeds precultured for 0 and 4 days yielded suitable embryonic leaves for bud induction.
- Taxon
- peanut
Arachis hypogaeat0 - Organ / stage
- embryonic leafe0
- Treatment
- seeds precultured for 0 and 4 dayse0
- Outcome
- differentiation ratee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the embryonic leaf derived from seeds precultured for 0 and 4 days were suitable for the induction of adventitious bud for higher differentiation rate”
t0“peanut”
Claim · model-written summary
The embryonic leaf section near the petiole had higher bud differentiation than the distant section.
- Taxon
- peanutArachis hypogaeat0
- Organ / stage
- embryonic leaves near petiolee0
- Outcome
- differentiation rate of adventitious bude0
- Direction
- highere0
- Comparator
- portion far away from petiolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The differentiation rate of adventitious bud derived from 1 / 3 cross-section of embryonic leaves near petiole was higher than of the portion far away from petiole”
t0“peanut”
Claim · model-written summary
For Guihua 22, 40 g/L sugar was best for adventitious bud induction.
- Taxon
- peanutArachis hypogaeat0
- Material
- Guihua 22e0
- Treatment
- 40 and 50g / L sugar in MS mediume0
- Outcome
- induction of adventitious bude0
- Direction
- best suitede0
- Qualifier
- 40 and 50g / Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“40 and 50g / L sugar in MS medium was best suited for the induction of adventitious bud derived from embryonic leaves of Guihua 22 and Guihua 17,respectively”
t0“peanut”
Claim · model-written summary
For Guihua 17, 50 g/L sugar was best for adventitious bud induction.
- Taxon
- peanutArachis hypogaeat0
- Material
- Guihua 17e0
- Treatment
- 40 and 50g / L sugar in MS mediume0
- Outcome
- induction of adventitious bude0
- Direction
- best suitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“40 and 50g / L sugar in MS medium was best suited for the induction of adventitious bud derived from embryonic leaves of Guihua 22 and Guihua 17,respectively”
t0“peanut”
Claim · model-written summary
The 4 mg/L 6-BA medium supported 100% bud differentiation in Guihua 17.
- Taxon
- peanutArachis hypogaeat0
- Material
- Guihua 17e0
- Treatment
- MS + 4 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3e0
- Outcome
- differentiation ratee0
- Direction
- beneficiale0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium MS + 4 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 and MS + 6 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 was beneficial for the differentiation of adventitious buds in Guihua 17 and Guihua 22,respectively,with 100% of differentiation rate”
t0“peanut”
Claim · model-written summary
The 6 mg/L 6-BA medium supported 100% bud differentiation in Guihua 22.
- Taxon
- peanutArachis hypogaeat0
- Material
- Guihua 22e0
- Treatment
- MS + 6 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3e0
- Outcome
- differentiation ratee0
- Direction
- beneficiale0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium MS + 4 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 and MS + 6 mg / L 6-BA + 1 mg / L NAA + 2 mg / L AgNO3 was beneficial for the differentiation of adventitious buds in Guihua 17 and Guihua 22,respectively,with 100% of differentiation rate”
t0“peanut”
Claim · model-written summary
The specified subculture medium promoted bud growth and plantlet development.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- MS + 1.5 mg / L 6-BA + 0.4 mg / L NAAe0
- Outcome
- growth of adventitious buds and development of plantletse0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The subculture medium MS + 1.5 mg / L 6-BA + 0.4 mg / L NAA was suitable for promoting the growth of adventitious buds and development of plantlets”
t0“peanut”
Claim · model-written summary
The half-strength MS medium was best for plantlet rooting.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- 1 / 2 MS + 0.5 mg / L 6-BA + 2.0 mg / L NAAe0
- Outcome
- rooting of plantlete0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best medium for rooting of plantlet was 1 / 2 MS + 0.5 mg / L 6-BA + 2.0 mg / L NAA”
t0“peanut”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.