Abstract
Two phenylethanoid glycosides(PhGs), plantamajoside and acteoside, were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography(HPCPC) with ethyl acetate, n-butanol, ethanol and water(0.5:0.5:0.1:1, volume ratio) as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of >93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry(ESI-MS n ) in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the
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Claims 4
Claim · model-written summary
Plantamajoside was isolated from the aerial parts of Plantago asiatica for the first time.
- Taxon
- Plantago asiatica
Plantago asiaticae0 - Organ / stage
- aerial partse0
- Outcome
- plantamajosidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantamajoside and acteoside, were isolated and purified from the aerial parts of Plantago asiatica for the first time”
Claim · model-written summary
Acteoside was isolated from the aerial parts of Plantago asiatica for the first time.
- Taxon
- Plantago asiaticaPlantago asiaticae0
- Organ / stage
- aerial partse0
- Outcome
- acteosidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantamajoside and acteoside, were isolated and purified from the aerial parts of Plantago asiatica for the first time”
Claim · model-written summary
The extraction purified 45.6 mg of plantamajoside from 1341 mg of n-butanol extract.
- Taxon
- P. asiaticaPlantago asiaticae0
- Outcome
- plantamajosidee0
- Value
- 45.6 mge0
- Qualifier
- 1341 mg of the n-butanol extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of >93.3%”
Claim · model-written summary
The extraction purified 293.8 mg of acteoside from 1341 mg of n-butanol extract.
- Taxon
- P. asiaticaPlantago asiaticae0
- Outcome
- acteosidee0
- Value
- 293.8 mge0
- Qualifier
- with a purity of >93.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of >93.3%”