Abstract
The multiplex RT-PCR assay was established to simultaneously detect Apple stem grooving virus(ASGV) ,Apple chlorotic leaf spot virus(ACLSV) and Apple stem pitting virus(ASPV) . Using tissue culture apple seedling(Wangshanhong) infected by three latent apple viruses as sample,a series of factors affecting the multiplex RT-PCR were optimized. Sensibility test showed that it could simultaneously detect out ASGV,ACLSV and ASPV in 187.5 ng total RNA. The multiplex RT-PCR products were cloned and sent for sequencing,and the sequences show high homologies to the published virus sequences. Consistent results for virus detection of 12 apple samples in field were obtained by the multiplex RT-PCR and single RT-PCR system,suggesting the accuracy of the system in ASGV,ACLSV and ASPV detection.
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Claims 3
Claim · model-written summary
The multiplex RT-PCR simultaneously detected ASGV, ACLSV and ASPV in 187.5 ng total RNA from infected tissue culture apple seedlings.
- Taxon
- apple
Malus domesticae0 - Outcome
- simultaneously detect out ASGV,ACLSV and ASPVe1
- Value
- 187.5 ng total RNAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using tissue culture apple seedling(Wangshanhong) infected by three latent apple viruses as sample”
e1“it could simultaneously detect out ASGV,ACLSV and ASPV in 187.5 ng total RNA”
Claim · model-written summary
Sequencing of the cloned multiplex RT-PCR products showed high homology to published virus sequences.
Verbatim
e2“the sequences show high homologies to the published virus sequences”
Claim · model-written summary
The multiplex RT-PCR gave results consistent with single RT-PCR for virus detection in 12 field apple samples, suggesting accuracy for ASGV, ACLSV and ASPV detection.
- Taxon
- appleMalus domesticae3
- Outcome
- Consistent results for virus detectione3
- Comparator
- single RT-PCR systeme3
- Setting
- fielde3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Consistent results for virus detection of 12 apple samples in field were obtained by the multiplex RT-PCR and single RT-PCR system”