Abstract
Partially purified preparations of lipoxygenase from the germinating barley embryos converted linoleic acid to 9- and 13-hydroperoxy linoleic acids in the ratio of approximately 3:1, while the similar preparations from the ungerminated embryos converted linoleic acid mainly to 9-hydroperoxy linoleic acid. Isoelectric focusing of the partially purified preparations of the germinating embryos revealed the presence of the two lipoxygenase active peaks, having isoelectric point at pH 4.9 and 6.6, respectively. The former peak (barley lipoxygenase-1) was identical to lipoxygenase of the ungerminated embryos, but the latter peak (barley lipoxygenase-2) was found only in the germinating embryos. The newly found isoenzyme, barley lipoxygenase-2, converted linoleic acid mainly to 13-hydroperoxy linoleic acid, and could oxidize esterified derivatives of linoleic acid (methyl linoleate and trilinolein) much strongly than barley lipoxygenase-1.
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Claims 7
Claim · model-written summary
Germinating barley embryo preparations converted linoleic acid to 9- and 13-hydroperoxy linoleic acids at about 3:1.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- germinating barley embryose0
- Gene
- lipoxygenasee0
- Outcome
- 9- and 13-hydroperoxy linoleic acidse0
- Value
- approximately 3:1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Partially purified preparations of lipoxygenase from the germinating barley embryos converted linoleic acid to 9- and 13-hydroperoxy linoleic acids in the ratio of approximately 3:1”
Claim · model-written summary
Ungerminated barley embryo preparations converted linoleic acid mainly to 9-hydroperoxy linoleic acid.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- ungerminated embryose0
- Gene
- lipoxygenaset0
- Outcome
- 9-hydroperoxy linoleic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the similar preparations from the ungerminated embryos converted linoleic acid mainly to 9-hydroperoxy linoleic acid”
t0“lipoxygenase isoenzyme in germinating barley embryos”
Claim · model-written summary
Germinating barley embryos showed two lipoxygenase active peaks at pH 4.9 and 6.6.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- germinating embryose0
- Outcome
- two lipoxygenase active peakse0
- Value
- pH 4.9 and 6.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“germinating embryos revealed the presence of the two lipoxygenase active peaks, having isoelectric point at pH 4.9 and 6.6”
t0“lipoxygenase isoenzyme in germinating barley embryos”
Claim · model-written summary
Barley lipoxygenase-1 was identical to the lipoxygenase of ungerminated embryos.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- ungerminated embryose0
- Gene
- barley lipoxygenase-1e0
- Outcome
- identical to lipoxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The former peak (barley lipoxygenase-1) was identical to lipoxygenase of the ungerminated embryos”
Claim · model-written summary
Barley lipoxygenase-2 occurred only in germinating embryos.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- germinating embryose0
- Gene
- barley lipoxygenase-2e0
- Outcome
- found only in the germinating embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the latter peak (barley lipoxygenase-2) was found only in the germinating embryos”
Claim · model-written summary
Barley lipoxygenase-2 converted linoleic acid mainly to 13-hydroperoxy linoleic acid.
- Taxon
- barleyHordeum vulgaree0
- Gene
- barley lipoxygenase-2e0
- Outcome
- 13-hydroperoxy linoleic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley lipoxygenase-2, converted linoleic acid mainly to 13-hydroperoxy linoleic acid”
Claim · model-written summary
Barley lipoxygenase-2 oxidized methyl linoleate and trilinolein more strongly than barley lipoxygenase-1.
- Taxon
- barleyHordeum vulgaret0
- Gene
- barley lipoxygenase-1e0
- Outcome
- oxidize esterified derivatives of linoleic acide0
- Direction
- much stronglye0
- Comparator
- barley lipoxygenase-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could oxidize esterified derivatives of linoleic acid (methyl linoleate and trilinolein) much strongly than barley lipoxygenase-1”
t0“lipoxygenase isoenzyme in germinating barley embryos”