Paper · 2005 · South African Journal of Botany

In vitro high frequency direct root and shoot regeneration in sweet potato using the ethylene inhibitor silver nitrate

Y. Gong; F. Gao; K. Tang; et al.

7
claims
14
verbatim quotes
31
citations
3
research areas
✓
Open access · cc-by

Abstract

The role of silver nitrate in the regulation of direct root and shoot regeneration from various explants of sweet potato (Ipomoea batatas L.) was studied. A rapid and high frequency direct shoot regeneration procedure was achieved from stem and lamina explants. The magnitude of the response to silver nitrate varied among different explants and silver nitrate was found to have a significant effect on shoot regeneration. The best shoot regeneration was achieved from stem explants cultured on basal MS medium supplemented with 1.0mg l− 1 NAA without silver nitrate, with the regeneration frequency reaching 86.3%. With the addition of 8.0mg l− 1 silver nitrate on MS medium, 73.3% of lamina explants could directly regenerate shoots. Our study suggests that the addition of silver nitrate can promote shoot regeneration from various explants. The established procedure enables the production of a large number of plantlets within a short time, and thus can be used for assisting conventional breeding programmes and gene transfer studies.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Stem and lamina explants yielded rapid, high frequency direct shoot regeneration.

Taxon
sweet potatoIpomoea batatase1
Organ / stage
stem and lamina explantse0
Outcome
direct shoot regeneratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A rapid and high frequency direct shoot regeneration procedure was achieved from stem and lamina explants.”

✓ located verbatim in the source · abstract, characters 155–261 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Response to silver nitrate varied among explant types.

Taxon
sweet potatoIpomoea batatase1
Organ / stage
different explantse0
Outcome
response to silver nitratee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The magnitude of the response to silver nitrate varied among different explants”

✓ located verbatim in the source · abstract, characters 262–341 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Silver nitrate significantly affected shoot regeneration.

Taxon
sweet potatoIpomoea batatase1
Treatment
silver nitratee0
Outcome
shoot regeneratione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“silver nitrate was found to have a significant effect on shoot regeneration”

✓ located verbatim in the source · abstract, characters 346–421 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Stem explants on basal MS medium with 1.0mg l− 1 NAA and no silver nitrate had the best shoot regeneration, reaching 86.3%.

Taxon
sweet potatoIpomoea batatase1
Organ / stage
stem explantse0
Treatment
basal MS medium supplemented with 1.0mg l− 1 NAA without silver nitratee0
Outcome
shoot regeneratione0
Direction
beste0
Value
86.3%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The best shoot regeneration was achieved from stem explants cultured on basal MS medium supplemented with 1.0mg l− 1 NAA without silver nitrate, with the regeneration frequency reaching 86.3%.”

✓ located verbatim in the source · abstract, characters 423–615 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

On MS medium with 8.0mg l− 1 silver nitrate, 73.3% of lamina explants directly regenerated shoots.

Taxon
sweet potatoIpomoea batatase1
Organ / stage
lamina explantse0
Treatment
8.0mg l− 1 silver nitrate on MS mediume0
Outcome
directly regenerate shootse0
Value
73.3%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“With the addition of 8.0mg l− 1 silver nitrate on MS medium, 73.3% of lamina explants could directly regenerate shoots.”

✓ located verbatim in the source · abstract, characters 616–735 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest that silver nitrate can promote shoot regeneration from various explants.

Taxon
sweet potatoIpomoea batatase1
Organ / stage
various explantse0
Treatment
addition of silver nitratee0
Outcome
promote shoot regeneratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the addition of silver nitrate can promote shoot regeneration from various explants”

✓ located verbatim in the source · abstract, characters 760–843 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The established regeneration procedure enables production of many plantlets quickly for breeding and gene transfer studies.

Taxon
sweet potatoIpomoea batatase1
Outcome
production of a large number of plantlets within a short timee0
Qualifier
assisting conventional breeding programmes and gene transfer studiese0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ipomoea batatas
Ipomoea batatas · TaxonEarth100 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The established procedure enables the production of a large number of plantlets within a short time, and thus can be used for assisting conventional breeding programmes and gene transfer studies.”

✓ located verbatim in the source · abstract, characters 845–1040 · show in text ↑
e1

“sweet potato (Ipomoea batatas L.)”

✓ located verbatim in the source · abstract, characters 108–141 · show in text ↑

silver(1+) nitrate · growth · Ipomoea batatas (sweet potato)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
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1-naphthaleneacetic acid · growth · Ipomoea batatas (sweet potato)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

10 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. positive

8 papers more in this group

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in vitro · growth · Ipomoea batatas (sweet potato)Positive and negative results

32 papers: 29 positive, 3 negative or no effect

28 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

28 papers more in this group

See all 32 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.