Abstract
The SPE-HPLC method was developed to determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6) in the bark of eight taxa (I-VIII) belonging to three species of the genus Salix and originating from a natural habitat or cultivated for pharmaceutical purposes. The chalcones were separated by HPLC under gradient elution with the concentration of ACN increasing from 20% to 50% in 0.1% aqueous H3PO, (tG 15 min). The content of both compounds was determined by an external standardization with the use of isoliquiritigenin (7) as a reference substance - a commercially available chalcone, and also isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6). The latter compound was isolated from the bark of Salix daphnoides (IV) by a CC and semi-preparative HPLC and its structure was elucidated by MS and NMR spectra. It was stated that 6"-O-p-coumaroylisosalipurposide (6), in addition to isosalipurposide (5), is a characteristic flavonoid for the S. daphnoides species. Moreover, the presence of these two chalcones was confirmed in the bark of S. acutifolia (I). Differences were observed in the results obtained from a quantitative analysis due to the type of reference substance used. The content of chalcones was varied and dependent on the species selected for analysis, namely from 22.01/21.08 mg/g in S. daphnoides clone 1095 (III) to 2.47/2.44 mg/g in S. daphnoides (II), collected from a natural habitat. Isosalipurposide (5) was determined in all the investigated species and clones of Salix, besides a number of naringenin derivatives. Separation of all flavonoids: flavanones - naringenin (1), naringenin (+)-5-O-glucoside (2), (-)-5-O-glucoside (3), 7-O-glucoside (4) and chalcones (5 and 6) was performed under gradient elution with the same solvents and changes in ACN concentration from 2% to 37% (tG 60 min). The total amount of flavanones ranged from 4.69 mg/g in S. purpurea clone 1132 (VII) to 41.93 mg/g in S. purpuea (VIII) from Herbapol Wrocław.
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Claims 12
Claim · model-written summary
SPE-HPLC was developed to determine isosalipurposide and its derivative in Salix bark.
- Organ / stage
- barke0
- Outcome
- determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6)e0
- Value
- eight taxae0
SalixOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salix”
e0“The SPE-HPLC method was developed to determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6) in the bark of eight taxa (I-VIII) belonging to three species of the genus Salix”
Claim · model-written summary
The derivative was isolated from Salix daphnoides bark and structurally identified by MS and NMR.
- Taxon
- Salix daphnoides
Salix daphnoidese0 - Organ / stage
- barke0
- Outcome
- structure was elucidated by MS and NMR spectrae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The latter compound was isolated from the bark of Salix daphnoides (IV) by a CC and semi-preparative HPLC and its structure was elucidated by MS and NMR spectra”
Claim · model-written summary
6"-O-p-coumaroylisosalipurposide is characteristic of S. daphnoides.
- Taxon
- S. daphnoidesSalix daphnoidese0
- Outcome
- 6"-O-p-coumaroylisosalipurposide (6)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6"-O-p-coumaroylisosalipurposide (6), in addition to isosalipurposide (5), is a characteristic flavonoid for the S. daphnoides species”
Claim · model-written summary
Isosalipurposide is characteristic of S. daphnoides.
- Taxon
- S. daphnoidesSalix daphnoidese0
- Outcome
- isosalipurposide (5)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in addition to isosalipurposide (5), is a characteristic flavonoid for the S. daphnoides species”
Claim · model-written summary
Both chalcones were found in S. acutifolia bark.
- Taxon
- S. acutifolia
Salix acutifoliae0 - Organ / stage
- barke0
- Outcome
- presence of these two chalconese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salix”
e0“the presence of these two chalcones was confirmed in the bark of S. acutifolia (I)”
Claim · model-written summary
Quantitative estimates differed with the reference substance used.
- Outcome
- Differences were observed in the results obtained from a quantitative analysise0
- Comparator
- type of reference substance usede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salix”
e0“Differences were observed in the results obtained from a quantitative analysis due to the type of reference substance used”
Claim · model-written summary
The higher reported chalcone content was 22.01/21.08 mg/g in S. daphnoides clone 1095.
- Taxon
- S. daphnoidesSalix daphnoidese0
- Material
- clone 1095e0
- Outcome
- content of chalconese0
- Direction
- variede0
- Value
- 22.01/21.08 mg/ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The content of chalcones was varied and dependent on the species selected for analysis, namely from 22.01/21.08 mg/g in S. daphnoides clone 1095 (III) to 2.47/2.44 mg/g in S. daphnoides (II)”
Claim · model-written summary
The lower reported chalcone content was 2.47/2.44 mg/g in wild S. daphnoides.
- Taxon
- S. daphnoidesSalix daphnoidese0
- Outcome
- content of chalconese0
- Direction
- variede0
- Value
- 2.47/2.44 mg/ge0
- Setting
- natural habitate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The content of chalcones was varied and dependent on the species selected for analysis, namely from 22.01/21.08 mg/g in S. daphnoides clone 1095 (III) to 2.47/2.44 mg/g in S. daphnoides (II), collected from a natural habitat”
Claim · model-written summary
Isosalipurposide and naringenin derivatives were detected across investigated Salix taxa and clones.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isosalipurposide (5) was determined in all the investigated species and clones of Salix, besides a number of naringenin derivatives”
Claim · model-written summary
The method separated four flavanones and two chalcones.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Salix”
e0“Separation of all flavonoids: flavanones - naringenin (1), naringenin (+)-5-O-glucoside (2), (-)-5-O-glucoside (3), 7-O-glucoside (4) and chalcones (5 and 6) was performed under gradient elution with the same solvents and changes in ACN concentration from 2% to 37% (tG 60 min)”
Claim · model-written summary
Total flavanones were 4.69 mg/g in S. purpurea clone 1132.
- Taxon
- S. purpurea
Salix purpureae0 - Material
- clone 1132e0
- Outcome
- total amount of flavanonese0
- Direction
- rangede0
- Value
- 4.69 mg/ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total amount of flavanones ranged from 4.69 mg/g in S. purpurea clone 1132 (VII) to 41.93 mg/g in S. purpuea (VIII) from Herbapol Wrocław”
Claim · model-written summary
Total flavanones were 41.93 mg/g in S. purpuea from Herbapol Wrocław.
Verbatim
e0“The total amount of flavanones ranged from 4.69 mg/g in S. purpurea clone 1132 (VII) to 41.93 mg/g in S. purpuea (VIII) from Herbapol Wrocław”