Abstract
Low molecular weight, cysteine-rich metal-binding peptides are produced in plants exposed to metals. This study focuses on the hypothesis that the rate of phytochelatin production is important to metal tolerance. Four clones of Deschampsia caespitosa tolerant of Cu and Ni, and one non-tolerant clone, were exposed to Cu and Ni for various times up to 24h. Root extracts were chromatographed on an anion exchange column to separate the metal-peptide fraction from unbound metal. In three metal-tolerant clones up to 95% of the buffer-soluble Cu and Ni was found in the metal-peptide fraction after 1h of exposure, with little change occurring subsequently. The non-tolerant clone bound only 2% of the metal after 1h. Copper remained low over 24h while Ni increased in the peptide fraction to the levels found in the tolerant clones. One metal tolerant clone did not conform to the patterns found in the other three. These data support the hypothesis that metal tolerance is, in some cases, related to the ability of roots to rapidly bind the metal that enters cells.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
In three metal-tolerant clones up to 95% of buffer-soluble Cu and Ni was in the metal-peptide fraction after 1 h of exposure, with little change afterwards.
Verbatim
e1“Four clones of Deschampsia caespitosa tolerant of Cu and Ni”
e2“In three metal-tolerant clones up to 95% of the buffer-soluble Cu and Ni was found in the metal-peptide fraction after 1h of exposure, with little change occurring subsequently”
Claim · model-written summary
The non-tolerant clone bound only 2% of the metal after 1 h.
Verbatim
e1“Four clones of Deschampsia caespitosa tolerant of Cu and Ni”
e3“The non-tolerant clone bound only 2% of the metal after 1h”
Claim · model-written summary
Copper in the peptide fraction remained low over 24 h of exposure.
Verbatim
e1“Four clones of Deschampsia caespitosa tolerant of Cu and Ni”
e4“Copper remained low over 24h while Ni increased in the peptide fraction to the levels found in the tolerant clones”
Claim · model-written summary
Ni in the peptide fraction increased over 24 h to the levels found in the tolerant clones.
Verbatim
e1“Four clones of Deschampsia caespitosa tolerant of Cu and Ni”
e4“Copper remained low over 24h while Ni increased in the peptide fraction to the levels found in the tolerant clones”
Claim · model-written summary
Metal tolerance is, in some cases, related to the ability of roots to rapidly bind the metal that enters cells.
Not reportedDirection
Verbatim
e1“Four clones of Deschampsia caespitosa tolerant of Cu and Ni”
e5“metal tolerance is, in some cases, related to the ability of roots to rapidly bind the metal that enters cells”