Paper · 1997 · Biologia Plantarum

Effect of lead on nitrate reductase activity and alleviation of lead toxicity by inorganic salts and 6-benzylaminopurine

R.P. Singh; S. Dabas; A. Choudhary; et al.

11
claims
21
verbatim quotes
26
citations
1
research areas
✓
Open access

Abstract

A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337). Leaf NRA, on the other hand, increased significantly with increasing lead concentration which was more pronounced in cvs. MH8320 and ML337 than in cv. K851. Total nitrogen content of root and shoot was generally increased due to supply of the lead acetate, whereas the total nitrogen content of the colyledons was hardly affected in cvs. MH8320 and ML337 and decreased in cv. K851 during the early growth phase. The inhibition of root NRA could be alleviated by addition of inorganic salts (K2HPO4 and KNO3, but not CaCl2) or 6-benzylaminopurine (BAP) in the incubation medium. Lead mediated inhibition of root NRA was similar in light and dark grown seedlings, but lead induced increase in leaf NRA was more pronounced in the light than in the dark.

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Claims 11

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lead acetate decreased root nitrate reductase activity with concentration.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
0.1 to 2.0 mM lead acetatee0
Gene
nitrate reductasee0
Outcome
root nitrate reductase (E.C.1.6.6.1) activity (NRA)e0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Leaf NRA increased significantly as lead concentration increased, especially in MH8320 and ML337 compared with K851.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Material
MH8320 and ML337e1
Treatment
increasing lead concentratione1
Outcome
Leaf NRAe1
Direction
increasede1
Comparator
cv. K851e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Leaf NRA, on the other hand, increased significantly with increasing lead concentration which was more pronounced in cvs. MH8320 and ML337 than in cv. K851.”

✓ located verbatim in the source · abstract, characters 207–363 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Lead acetate generally increased root and shoot total nitrogen content.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
lead acetatee1
Outcome
Total nitrogen content of root and shoote1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Total nitrogen content of root and shoot was generally increased due to supply of the lead acetate, whereas the total nitrogen content of the colyledons was hardly affected in cvs. MH8320 and ML337 and decreased in cv. K851 during the early growth phase.”

✓ located verbatim in the source · abstract, characters 364–618 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cotyledon total nitrogen content was hardly affected in MH8320 and ML337.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Material
MH8320 and ML337e1
Treatment
lead acetatee1
Outcome
total nitrogen content of the colyledonse1
Direction
hardly affectede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Total nitrogen content of root and shoot was generally increased due to supply of the lead acetate, whereas the total nitrogen content of the colyledons was hardly affected in cvs. MH8320 and ML337 and decreased in cv. K851 during the early growth phase.”

✓ located verbatim in the source · abstract, characters 364–618 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cotyledon total nitrogen content decreased in K851 during early growth.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Material
K851e1
Organ / stage
early growth phasee1
Treatment
lead acetatee1
Outcome
total nitrogen content of the colyledonse1
Direction
decreasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Total nitrogen content of root and shoot was generally increased due to supply of the lead acetate, whereas the total nitrogen content of the colyledons was hardly affected in cvs. MH8320 and ML337 and decreased in cv. K851 during the early growth phase.”

✓ located verbatim in the source · abstract, characters 364–618 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

K2HPO4 alleviated lead inhibition of root NRA.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
K2HPO4e1
Outcome
inhibition of root NRAe1
Direction
alleviatede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“The inhibition of root NRA could be alleviated by addition of inorganic salts (K2HPO4 and KNO3, but not CaCl2) or 6-benzylaminopurine (BAP) in the incubation medium.”

✓ located verbatim in the source · abstract, characters 619–784 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

KNO3 alleviated lead inhibition of root NRA.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
KNO3e1
Outcome
inhibition of root NRAe1
Direction
alleviatede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“The inhibition of root NRA could be alleviated by addition of inorganic salts (K2HPO4 and KNO3, but not CaCl2) or 6-benzylaminopurine (BAP) in the incubation medium.”

✓ located verbatim in the source · abstract, characters 619–784 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

6-benzylaminopurine (BAP) alleviated lead inhibition of root NRA.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
6-benzylaminopurine (BAP)e1
Outcome
inhibition of root NRAe1
Direction
alleviatede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“The inhibition of root NRA could be alleviated by addition of inorganic salts (K2HPO4 and KNO3, but not CaCl2) or 6-benzylaminopurine (BAP) in the incubation medium.”

✓ located verbatim in the source · abstract, characters 619–784 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

CaCl2 did not alleviate lead inhibition of root NRA.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
CaCl2e1
Outcome
inhibition of root NRAe1
Direction
note1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“The inhibition of root NRA could be alleviated by addition of inorganic salts (K2HPO4 and KNO3, but not CaCl2) or 6-benzylaminopurine (BAP) in the incubation medium.”

✓ located verbatim in the source · abstract, characters 619–784 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Lead inhibition of root NRA was similar in light and dark grown seedlings.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
Leade1
Outcome
inhibition of root NRAe1
Direction
similare1
Comparator
light and dark grown seedlingse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Lead mediated inhibition of root NRA was similar in light and dark grown seedlings, but lead induced increase in leaf NRA was more pronounced in the light than in the dark.”

✓ located verbatim in the source · abstract, characters 785–957 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Lead induced increase in leaf NRA was more pronounced in light than dark.

Taxon
Vigna radiata (L.) WilczekVigna radiatae0
Treatment
leade1
Outcome
increase in leaf NRAe1
Direction
more pronouncede1
Qualifier
the lighte1
Comparator
the darke1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A concentration dependent decrease in root nitrate reductase (E.C.1.6.6.1) activity (NRA) by 0.1 to 2.0 mM lead acetate was noticed in three cultivars of Vigna radiata (L.) Wilczek (K851, MH8320 and ML337).”

✓ located verbatim in the source · abstract, characters 0–206 · show in text ↑
e1

“Lead mediated inhibition of root NRA was similar in light and dark grown seedlings, but lead induced increase in leaf NRA was more pronounced in the light than in the dark.”

✓ located verbatim in the source · abstract, characters 785–957 · show in text ↑

N-benzyladenine · disease · Vigna radiata (mung bean)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.