Paper · 2001 · Biologia Plantarum

The Response of Nitrate Reductase Activity and Nitrate Assimilation in Maize Roots to Growth Regulators at Acidic pH

N. Shankar; S.R. Khan; H.S. Srivastava

11
claims
23
verbatim quotes
11
citations
1
research areas
✓
Open access

Abstract

Nitrate and total nitrogen contents, and nitrate reductase (NR) activity of the excised maize roots in buffered or unbuffered nitrate solution (at pH 6.5 or 4.5) as affected by putrescine (PUT), abscisic acid (ABA) and salicylic acid (SA) were investigated. In unbufferred solution, the NR activity was lower at pH 4.5 as compared to that at pH 6.5, but in bufferred solution the activity was higher at lower pH. Supply of 100 µM PUT or 500 µM SA, promoted NR activity and 50 µM ABA inhibited the activity at pH 6.5. However, at pH 4.5, PUT and SA inhibited NR activity and ABA had no effect. In most cases, the increase in NR activity was positively correlated with total organic nitrogen and a negatively with nitrate content. A reverse situation was found when NR activity was inhibited by the growth regulators.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

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Claims 11

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

In unbuffered solution, nitrate reductase activity was lower at pH 4.5 than pH 6.5.

Taxon
maizeZea mayse1
Organ / stage
rootse1
Treatment
pH 4.5e0
Outcome
NR activitye0
Direction
lowere0
Qualifier
unbufferred solutione0
Comparator
pH 6.5e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In unbufferred solution, the NR activity was lower at pH 4.5 as compared to that at pH 6.5”

✓ located verbatim in the source · abstract, characters 258–348 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

In buffered solution, nitrate reductase activity was higher at lower pH.

Taxon
maizeZea mayse1
Treatment
lower pHe0
Outcome
NR) activitye1
Direction
highere0
Qualifier
bufferred solutione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“in bufferred solution the activity was higher at lower pH”

✓ located verbatim in the source · abstract, characters 354–411 · show in text ↑
e1

“Nitrate and total nitrogen contents, and nitrate reductase (NR) activity of the excised maize roots”

✓ located verbatim in the source · abstract, characters 0–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

100 µM PUT promoted nitrate reductase activity at pH 6.5.

Taxon
maizeZea mayse1
Treatment
100 µM PUTe0
Outcome
NR activitye0
Direction
promotede0
Qualifier
pH 6.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Supply of 100 µM PUT or 500 µM SA, promoted NR activity and 50 µM ABA inhibited the activity at pH 6.5.”

✓ located verbatim in the source · abstract, characters 413–516 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

500 µM SA promoted nitrate reductase activity at pH 6.5.

Taxon
maizeZea mayse1
Treatment
500 µM SAe0
Outcome
NR activitye0
Direction
promotede0
Qualifier
pH 6.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Supply of 100 µM PUT or 500 µM SA, promoted NR activity and 50 µM ABA inhibited the activity at pH 6.5.”

✓ located verbatim in the source · abstract, characters 413–516 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

50 µM ABA inhibited nitrate reductase activity at pH 6.5.

Taxon
maizeZea mayse1
Treatment
50 µM ABAe0
Outcome
NR activitye0
Direction
inhibitede0
Qualifier
pH 6.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Supply of 100 µM PUT or 500 µM SA, promoted NR activity and 50 µM ABA inhibited the activity at pH 6.5.”

✓ located verbatim in the source · abstract, characters 413–516 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

PUT inhibited nitrate reductase activity at pH 4.5.

Taxon
maizeZea mayse1
Treatment
PUTe0
Outcome
NR activitye0
Direction
inhibitede0
Qualifier
pH 4.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“at pH 4.5, PUT and SA inhibited NR activity and ABA had no effect”

✓ located verbatim in the source · abstract, characters 526–591 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

SA inhibited nitrate reductase activity at pH 4.5.

Taxon
maizeZea mayse1
Treatment
SAe0
Outcome
NR activitye0
Direction
inhibitede0
Qualifier
pH 4.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“at pH 4.5, PUT and SA inhibited NR activity and ABA had no effect”

✓ located verbatim in the source · abstract, characters 526–591 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

ABA had no effect on nitrate reductase activity at pH 4.5.

Taxon
maizeZea mayse1
Treatment
ABAe0
Outcome
NR activitye0
Direction
no effecte0
Qualifier
pH 4.5e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“at pH 4.5, PUT and SA inhibited NR activity and ABA had no effect”

✓ located verbatim in the source · abstract, characters 526–591 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

In most cases increased nitrate reductase activity correlated positively with total organic nitrogen.

Taxon
maizeZea mayse1
Outcome
NR activitye0
Direction
positivelye0
Qualifier
In most casese0
Comparator
total organic nitrogene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In most cases, the increase in NR activity was positively correlated with total organic nitrogen and a negatively with nitrate content.”

✓ located verbatim in the source · abstract, characters 593–728 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Increased nitrate reductase activity correlated negatively with nitrate content.

Taxon
maizeZea mayse1
Outcome
NR activitye0
Direction
negativelye0
Comparator
nitrate contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In most cases, the increase in NR activity was positively correlated with total organic nitrogen and a negatively with nitrate content.”

✓ located verbatim in the source · abstract, characters 593–728 · show in text ↑
e1

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The opposite nitrogen relationships occurred when growth regulators inhibited nitrate reductase activity.

Taxon
maizeZea mayse2
Treatment
growth regulatorse0
Outcome
total organic nitrogene1
Qualifier
A reverse situatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A reverse situation was found when NR activity was inhibited by the growth regulators.”

✓ located verbatim in the source · abstract, characters 729–815 · show in text ↑
e1

“In most cases, the increase in NR activity was positively correlated with total organic nitrogen and a negatively with nitrate content.”

✓ located verbatim in the source · abstract, characters 593–728 · show in text ↑
e2

“excised maize roots”

✓ located verbatim in the source · abstract, characters 80–99 · show in text ↑

(+)-abscisic acid · physiology: enzyme activity · Zea mays (maize)Positive and negative results

8 papers: 5 positive, 3 negative or no effect

2 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.